Using morphological traits and molecular-genetic research methods, the authors have identified 24 species of naked amoeba from natural biotopes. The 18S rRNA gene sequences were obtained for the following species of naked amoeba: Amoeba proteus isolate AP07 (ON907618), Saccamoeba limax isolate SLU_22 (OP894078), Saccamoeba limax isolate SL_Uk19 (OQ520144), Saccamoeba sp. strain IDL777 (MZ079370), Thecamoeba striata isolate THS19 (OQ134482), Thecamoeba striata isolate THS20 (OQ134483), Thecamoeba similis isolate Prut river (OL604177), Thecamoeba similis isolate Baggersee Innsbruck (Baggersee Rossau) (OL604178), Thecamoeba quadrilineata isolate THQD2 (ON398269), Thecamoeba quadrilineata isolate THQA1 (ON398268), Thecamoeba sp. strain THS203 (MZ079371), Stenamoeba stenopodia isolate UKSS7 (OP375108), Stenamoeba stenopodia isolate POLSS7 (OP419588), Korotnevella stella isolate KSD2 (ON398267), Korotnevella stella isolate KSA1 (ON398266), Vexillifera bacillipedes isolate river Dnepr (OK649262), Vannella lata isolate Kamenka river (OL305063), Vannella lata isolate Varta river (OL305064), Vannella sp. strain VLS303 (MZ079372), Vannella simplex isolate Black Sea (OM403052), Vannella simplex isolate Mediterranean Sea (OM403053), Ripella sp. strain RPL100 (MZ079369), Mayorella vespertilioides isolate MV_7 (OP739500), Mayorella sp. isolate MY_7 (OP729930), Acanthamoeba sp. strain ATM123 (MZ079366), Acanthamoeba sp. isolate river Elbe (OK649261), Acanthamoeba polyphaga isolate AcPoly01 (ON908497), Acanthamoeba polyphaga isolate AcPoly15 (ON908496), Acanthamoeba griffini isolate Black sea (OM522832), Acanthamoeba griffini isolate Mediterranean Sea (OM522833), Cochliopodium actinophorum strain COP101 (MZ079367), Cochliopodium minus isolate river Stokhid (OK649264), Cochliopodium sp. strain COP102 (MZ079368), Vahlkampfia avara isolate VA7 (OP179657), Willaertia magna isolate river Teterev (OK649263). All of the naked amoebae species on the phylogenetic tree constructed based on the 18S rRNA gene are located within Amoebozoa and grouped with Tubulinea and Discosea. There are separate groups of freshwater, marine, and terrestrial biotopes; these groups are sister species relative to one another with low results of bootstrap analysis, which shows a low accuracy in the distances of particular amoeba species isolated from different natural biotopes.

作者利用形态特征和分子遗传学研究方法，从自然群落生境中鉴定出了 24 种裸阿米巴原虫。获得以下裸阿米巴物种的 18S rRNA 基因序列：变形虫分离株 AP07 (ON907618)、Saccamoeba limax分离株 SLU_22 (OP894078)、Saccamoeba limax分离株 SL_Uk19 (OQ520144)、Saccamoeba sp.。菌株 IDL777 (MZ079370)、Thecamoeba striata分离物 THS19 (OQ134482)、Thecamoeba striata分离物 THS20 (OQ134483)、Thecamoeba striata分离物 Prut River (OL604177)、Thecamoeba striata分离物 Baggersee Innsbruck (Baggersee Rossau) (OL604178)、Thecam四线阿米巴分离株 THQD2 (ON398269 )，Thecamoebaquadrilineata分离物 THQA1 (ON398268)，Thecamoeba sp。菌株 THS203 (MZ079371)、Stenamoba stenopodia分离株 UKSS7 (OP375108)、Stenamoba stenopodia分离株 POLSS7 (OP419588)、Korotnevella stella分离株 KSD2 (ON398267)、Korotnevella stella分离株 KSA1 (ON398266)、Vexillifera bacillipedes分离河第聂伯河 (OK6) 49262),瓦内拉分离物Kamenka 河 (OL305063)、Vannela lata分离物 Varta 河 (OL305064)、Vannela sp。菌株 VLS303 (MZ079372)、Vannella simplex分离物黑海 (OM403052)、Vannella simplex分离物地中海 (OM403053)、Ripella sp。菌株 RPL100 (MZ079369)、Mayorella vespertilioides分离株 MV_7 (OP739500)、Mayorella sp。分离 MY_7 (OP729930)，棘阿米巴sp。菌株 ATM123 (MZ079366)，棘阿米巴属。易北河分离株 (OK649261)、多食棘阿米巴分离株 AcPoly01 (ON908497)、多食棘阿米巴分离株 AcPoly15 (ON908496)、黑海棘阿米巴分离株 (OM522832)、地中海棘阿米巴分离株 (OM522833)、 Cochliopodium actinophorum菌株 COP101 (MZ) 079367),轮足属减去隔离河 Stokhid (OK649264)，Cochliopodium sp。菌株 COP102 (MZ079368)、Vahlkampfia avara分离株 VA7 (OP179657)、Willaertia magna隔离捷捷列夫河（OK649263）。基于18S rRNA基因构建的系统发育树上的所有裸变形虫物种都位于变形虫亚门内，并与管虫亚纲和圆盘亚纲分在一起。淡水、海洋和陆地生物生境分为不同的组别；这些群体彼此是姊妹物种，引导分析结果较低，这表明从不同自然生境中分离出的特定变形虫物种的距离准确度较低。