Premature ovarian insufficiency (POI) is defined as the development of hypergonadotropic hypogonadism before the age of 40 with definitive treatment being absent. In the current study, we aim to compare the efficacy of the cell sheet method with an intravenous (IV) application of adipose-derived mesenchymal stem cells (AdMSCs) to the POI with an animal model. In the current prospective study, 6-to-8-week-old Sprague Dawley rats were generated four groups: (i) a control group in which only PBS was administered; (ii) an only-POI group generated by cyclophosphamide; (iii) a POI group treated by way of IV AdMSCs; and (iv) a POI group treated by way of the cell sheet method. Twenty-eight days after an oophorectomy was performed, intracardiac blood was taken. Follicle count, immunohistochemical examination for GDF9, BMP15, and TUNEL were conducted, gene expressions of GDF9 and BMP15 were examined, and E2 was measured in the serum samples. With hematoxylin-eosin, in the third group, multi oocytes follicles were the most remarkable finding. In the fourth group, most of the follicles presented normal morphology. GDF9 involvement was similar between the first and fourth groups. BMP-15 immunoreactivity, in contrast to fourth group, was weak in all stages in the second and third groups. The current attempt represents a pioneer study in the literature in which a cell sheet method is used for the first time in a POI model. These results suggest that the cell sheet method may be a feasible and efficient method for the stem cell treatment of models with POI and could be a new treatment approach in POI.

卵巢早衰 (POI) 被定义为 40 岁之前出现高促性腺激素性性腺功能减退症，且缺乏明确的治疗。在当前的研究中，我们的目的是通过动物模型比较细胞片法与静脉注射（IV）应用脂肪间充质干细胞（AdMSCs）至 POI 的功效。在当前的前瞻性研究中，将 6 至 8 周龄的 Sprague Dawley 大鼠分为四组：(i) 仅给予 PBS 的对照组；(ii)由环磷酰胺产生的仅-POI基团；(iii) 通过 IV AdMSC 治疗的 POI 组；(iv)通过细胞片层法处理的POI组。进行卵巢切除术二十八天后，采集心内血液。对血清样本进行卵泡计数、GDF9、BMP15、TUNEL免疫组化检查，检测GDF9、BMP15基因表达，并测定E2。使用苏木精-伊红，在第三组中，最显着的发现是多卵母细胞卵泡。在第四组中，大多数卵泡呈现正常形态。第一组和第四组之间的 GDF9 参与程度相似。与第四组相比，第二组和第三组的 BMP-15 免疫反应性在所有阶段都很弱。当前的尝试代表了文献中的开创性研究，其中细胞片层方法首次在 POI 模型中使用。这些结果表明，细胞片法可能是一种可行且有效的干细胞治疗 POI 模型的方法，并可能成为 POI 的一种新的治疗方法。