Radiation-induced gastrointestinal damage is a common acute radiation syndrome. Previous studies have highlighted that Galectin-1 and Interleukin-6 (IL-6) are associated with flaking of small intestinal villi and intestinal radioresistance. Therefore, our goal is to study whether gut bacteria regulated by galectin-1 or IL-6 can mitigate radiation-induced small intestine damage. In this study, differences between galectin-1, sgp130-regulated and wild-type (WT) mice were analyzed by microbiome array. The effects of the Firmicutes/Bacteroidetes (F/B) ratio and the proportion of bacterial distribution at the phylum level were observed after 18 Gy whole abdomen radiation. Fecal microbiota transplantation was used to implant radioresistant gut flora into WT mice, and the number of viable small intestinal crypt foci was observed by immunohistochemistry. Fecal transplantation from galectin-1 knockout and sgp130 transgenic mice, with higher radiation resistance, into WT mice significantly increased the number of surviving small intestinal crypts. This radiation resistance, generated through gene regulation, was not affected by the F/B ratio. We initially found that the small intestinal villi of WT mice receiving radioresistant mouse fecal bacteria demonstrated better repair outcomes after radiation exposure. These results indicate the need for a focus on the identification and application of superior radioresistant bacterial strains. In our laboratory, we will further investigate specific radioresistant bacterial strains to alleviate acute side effects of radiation therapy to improve the patients' immune ability and postoperative quality of life.

中文翻译：

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微生物群在辐射引起的小肠损伤中的作用。

放射引起的胃肠道损伤是一种常见的急性放射综合征。先前的研究强调，Galectin-1 和 Interleukin-6 (IL-6) 与小肠绒毛剥落和肠道放射抗性有关。因此，我们的目标是研究半乳糖凝集素1或IL-6调节的肠道细菌是否可以减轻辐射引起的小肠损伤。在本研究中，通过微生物组阵列分析了 galectin-1、sgp130 调节的小鼠和野生型 (WT) 小鼠之间的差异。观察18 Gy全腹部辐射后对厚壁菌门/拟杆菌门(F/B)比值和门水平细菌分布比例的影响。采用粪便微生物移植将抗辐射肠道菌群植入WT小鼠体内，通过免疫组化法观察存活小肠隐窝病灶的数量。将半乳糖凝集素 1 敲除和 sgp130 转基因小鼠（具有更高的辐射抗性）的粪便移植到 WT 小鼠中，可显着增加存活小肠隐窝的数量。这种通过基因调控产生的辐射抗性不受 F/B 比率的影响。我们最初发现，接受辐射抗性小鼠粪便细菌的 WT 小鼠的小肠绒毛在辐射暴露后表现出更好的修复结果。这些结果表明需要重点关注优良耐辐射细菌菌株的鉴定和应用。在我们实验室，我们将进一步研究特定的抗辐射菌株，以减轻放射治疗的急性副​​作用，提高患者的免疫能力和术后生活质量。