METHODS The effect of hCOLII on proliferation of EA.hy926 cells was detected by MTT assay, the effect of hCOLII on cell migration was investigated by scratch assay, the effect of hCOLIII on cell cycle and apoptosis was detected by flow cytometry, the ability of hCOLII to induce angiogenesis of EA.hy926 cells was evaluated by angiogenesis assay, and the effect of hCOLII on VEGF expression was detected by RT-PCR analysis. RESULTS The hCOLIII at concentrations of 0.5mg/ml, 0.25 mg/ml and 0.125 mg/ml all showed certain effects on the proliferation and migration of human vascular endothelial cells. It could also affect the cell cycle, increase the PI index and increase the expression level of VEGF in human vascular endothelial cells. In the meantime, hCOLIII at the concentration of 0.5mg/ml also showed a promoting effect on vessel formation. CONCLUSIONS The hCOLIII has the potential to promote the endothelization process of blood vessels, mainly through affecting the proliferation, migration, and vascular-like structure of human endothelial cells. At the same time, hCOLIII can promote the expression of VEGF. This collagen demonstrated its potential as a raw material for cardiovascular implants.

中文翻译：

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Ⅲ型重组人源化胶原对人血管内皮细胞作用的研究

方法采用MTT法检测hCOLII对EA.hy926细胞增殖的影响，划痕实验检测hCOLII对细胞迁移的影响，流式细胞术检测hCOLIII对细胞周期和凋亡的影响，hCOLII对细胞周期和凋亡的影响。通过血管生成实验评估hCOLII对EA.hy926细胞血管生成的诱导作用，并通过RT-PCR分析检测hCOLII对VEGF表达的影响。结果0.5mg/ml、0.25mg/ml、0.125mg/ml浓度的hCOLIII均对人血管内皮细胞的增殖和迁移有一定的影响。它还可以影响细胞周期，提高PI指数，增加人血管内皮细胞中VEGF的表达水平。同时，0.5mg/ml浓度的hCOLIII也表现出对血管形成的促进作用。结论 hCOLIII具有促进血管内皮化过程的潜力，主要通过影响人内皮细胞的增殖、迁移和血管样结构来实现。同时，hCOLIII可以促进VEGF的表达。这种胶原蛋白展示了其作为心血管植入物原材料的潜力。