Polycystic ovary syndrome (PCOS) is an endocrine disorder, affecting women of child-bearing age, and the incidence rate is growing and assuming epidemic proportions. The etiology of PCOS remains unknown and there is no cure. Some animal models for PCOS have been established which have enhanced our understanding of the underlying mechanisms, but omics data for revealing PCOS pathogenesis and for drug discovery are still lacking. In the present study, proteomics analysis was used to construct a protein profile of the ovaries in a PCOS mouse model. The result showed a clear difference in protein profile between the PCOS and control group, with 495 upregulated proteins and 404 downregulated proteins in the PCOS group. The GO term and KEGG pathway analyses of differentially expressed proteins mainly showed involvement in lipid metabolism, oxidative stress, and immune response, which are consistent with pathological characteristics of PCOS in terms of abnormal metabolism, endocrine disorders, chronic inflammation and imbalance between oxidant and antioxidant levels. Also, we found that inflammatory responses were activated in the PCOS ovarium, while lipid biosynthetic process peroxisome, and bile secretion were inhibited. In addition, we found some alteration in unexpected pathways, such as glyoxylate and dicarboxylate metabolism, which should be investigated. The present study makes an important contribution to the current lack of PCOS ovarian proteomic data and provides an important reference for research and development of effective drugs and treatments for PCOS.

中文翻译：

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多囊卵巢综合征小鼠模型中蛋白质谱的显着改变

多囊卵巢综合症（PCOS）是一种影响育龄妇女的内分泌疾病，其发病率不断上升并呈流行病状。PCOS 的病因仍不清楚，也无法治愈。一些 PCOS 动物模型的建立增强了我们对其潜在机制的理解，但仍然缺乏揭示 PCOS 发病机制和药物发现的组学数据。在本研究中，蛋白质组学分析用于构建 PCOS 小鼠模型中卵巢的蛋白质谱。结果显示，PCOS 组和对照组之间的蛋白质谱存在明显差异，PCOS 组中有 495 个上调蛋白质和 404 个下调蛋白质。差异表达蛋白的GO term和KEGG通路分析主要参与脂质代谢、氧化应激、免疫反应等，与PCOS代谢异常、内分泌紊乱、慢性炎症、氧化与抗氧化失衡等病理特征一致。水平。此外，我们发现 PCOS 卵巢中的炎症反应被激活，而脂质生物合成过程过氧化物酶体和胆汁分泌则受到抑制。此外，我们发现了一些意想不到的途径的改变，例如乙醛酸和二羧酸代谢，应该进行研究。本研究为目前PCOS卵巢蛋白质组数据的缺乏做出了重要贡献，为研发PCOS有效药物和治疗方法提供了重要参考。