The study objective was to assess microbiological activity of sediments during the application of an EM preparation on the in Lake Skiertąg. Samples were taken before and after microbial bioremediation in the period 2020–2021 from five sediment layers. The abundance, biomass, and efficiency of the respiratory chain of sediment bacteria were determined by the direct methods in a fluorescence microscope. The rate of organic carbon oxidation was measured by the respiratory method. The abundance of benthic bacteria showed significant variability in the profile of each core, and decreased with an increase in sediment depth. The significant decrease in the number and biomass of bacteria in the sediment after the application of the EM technology, was observed during the second year of application of the preparation, and not immediately after its application. In the tested sediments, an significant increase in the percentage of cell with active dehydrogenases ETS+ in the total number of bacteria after application of the preparation was found, but at the same time significantly higher values of this parameter were demonstrated in 2021 compared to the first year of using microbial bioremediation. A significant positive correlation between the potential rate of organic carbon oxidation in the surface layer of sediments and the temperature in the water above the sediment indicates that the temperature conditions stimulated the respiration activity of the sediment microflora.

研究目的是评估在 Skiertąg 湖中施用 EM 制剂期间沉积物的微生物活性。样本是在 2020 年至 2021 年期间从五个沉积物层进行微生物生物修复之前和之后采集的。通过荧光显微镜直接法测定沉积物细菌呼吸链的丰度、生物量和效率。有机碳氧化率通过呼吸法测定。底栖细菌的丰度在每个岩心的剖面上表现出显着的变化，并随着沉积物深度的增加而减少。应用 EM 技术后，沉积物中细菌的数量和生物量显着下降，这是在应用制剂的第二年观察到的，而不是应用后立即观察到的。在测试的沉积物中，发现使用该制剂后，具有活性脱氢酶 ETS+ 的细胞占细菌总数的百分比显着增加，但同时与第一个相比，2021 年该参数的值显着更高使用微生物生物修复的年份。沉积物表层有机碳氧化的潜在速率与沉积物上方水温之间存在显着的正相关关系，表明温度条件刺激了沉积物微生物区系的呼吸活动。