Since the 1960 s, there has been a substantial amount of research directed towards investigating the biology of several types of stem cells, including embryonic stem cells, brain cells, and mesenchymal stem cells. In contemporary times, a wide array of stem cells has been utilized to treat several disorders, including bone marrow transplantation. In recent years, stem cell treatment has developed as a very promising and advanced field of scientific research. The progress of therapeutic methodologies has resulted in significant amounts of anticipation and expectation. Recently, there has been a notable proliferation of experimental methodologies aimed at isolating and developing stem cells, which have emerged concurrently. Stem cells possess significant vitality and exhibit vigorous proliferation, making them suitable candidates for in vitro modification. This article examines the progress made in stem cell isolation and explores several methodologies employed to promote the differentiation of stem cells. This study also explores the method of isolating bio-nanomaterials and discusses their viewpoint in the context of stem cell research. It also covers the potential for investigating stem cell applications in bioprinting and the usage of bionanomaterial in stem cell-related technologies and research. In conclusion, the review article concludes by highlighting the importance of incorporating state-of-the-art methods and technological breakthroughs into the future of stem cell research. Putting such an emphasis on constant innovation highlights the ever-changing character of science and the never-ending drive toward unlocking the maximum therapeutic potential of stem cells. This review would be a useful resource for researchers, clinicians, and policymakers in the stem cell research area, guiding the next steps in this fast-developing scientific concern.

自 20 世纪 60 年代以来，已有大量研究致力于研究几种类型干细胞的生物学特性，包括胚胎干细胞、脑细胞和间充质干细胞。在当代，多种干细胞已被用来治疗多种疾病，包括骨髓移植。近年来，干细胞治疗已发展成为一个非常有前途和先进的科学研究领域。治疗方法的进步引起了人们的广泛期待和期待。最近，旨在分离和发育干细胞的实验方法显着增加，这些方法同时出现。干细胞具有显着的活力并表现出旺盛的增殖能力，使其成为体外修饰的合适候选者。本文探讨了干细胞分离方面取得的进展，并探讨了用于促进干细胞分化的几种方法。这项研究还探讨了分离生物纳米材料的方法，并讨论了他们在干细胞研究背景下的观点。它还涵盖了研究干细胞在生物打印中的应用以及生物纳米材料在干细胞相关技术和研究中的使用的潜力。总之，这篇评论文章强调了将最先进的方法和技术突破纳入干细胞研究未来的重要性。对持续创新的重视凸显了科学不断变化的特征以及释放干细胞最大治疗潜力的永无止境的动力。这篇综述对于干细胞研究领域的研究人员、临床医生和政策制定者来说将是有用的资源，指导这一快速发展的科学问题的后续步骤。