Abstract—Currently, much attention in oncology is devoted to the issues of tumor heterogeneity, which creates serious problems in the diagnosis and therapy of malignant neoplasms. Intertumoral and intratumoral differences relate to various characteristics and aspects of the vital activity of tumor cells, including cellular metabolism. This review provides general information about the tumor metabolic heterogeneity with a focus on energy metabolism, its causes, mechanisms and research methods. Among the methods, fluorescence lifetime imaging is described in more detail as a new promising method for observing metabolic heterogeneity at the cellular level. The review demonstrates the importance of studying the features of tumor metabolism and identifying intra- and intertumoral metabolic differences.

摘要：目前，肿瘤异质性问题受到肿瘤学界的广泛关注，这给恶性肿瘤的诊断和治疗带来了严重的问题。肿瘤间和肿瘤内差异与肿瘤细胞生命活动的各种特征和方面有关，包括细胞代谢。本文综述了肿瘤代谢异质性的一般信息，重点关注能量代谢、其原因、机制和研究方法。在这些方法中，荧光寿命成像被更详细地描述为一种在细胞水平上观察代谢异质性的有前景的新方法。该综述证明了研究肿瘤代谢特征和识别肿瘤内和肿瘤间代谢差异的重要性。