Abstract—Hsp70 and hydrogen sulfide donors reduce inflammatory processes in human and animal cells. The biological action mediated by Hsp70 and H₂S donors (GYY4137 and sodium thiosulfate) depends on their protection kinetics from cell activation by lipopolysaccharides. However, the molecular mechanisms of action of Hsp70 and H₂S are not well understood. We studied the effect of human recombinant Hsp70 and H₂S donors on the formation of reactive oxygen species and tumor necrosis factor-alpha induced in human cells (THP-1) by lipopolysaccharides. Transcriptomic changes occurring in these cells after LPS administration in combination with GYY4137 pretreatment were investigated. The results we obtained showed that Hsp70 and hydrogen sulfide donors reduce inflammatory processes in cells activated by the action of LPS. Hsp70 and H₂S donors differed in the kinetics of the protective action, while hydrogen sulfide donors turned out to be more effective. The role of endocytosis in the mechanisms of protection of cells by H₂S and Hsp70 donors from the action of LPS was studied. It has been found that GYY4137 pretreatment of LPS-exposed cells reduces the LPS-induced induction of various pro-inflammatory genes and affects the expression of genes of various intracellular signaling pathways.

摘要—Hsp70 和硫化氢供体可减少人类和动物细胞的炎症过程。_{Hsp70 和 H 2} S 供体（GYY4137 和硫代硫酸钠）介导的生物作用取决于它们对脂多糖激活细胞的保护动力学。_{然而，Hsp70和H 2} S作用的分子机制尚不清楚。_{我们研究了人重组Hsp70和H 2} S供体对脂多糖诱导的人细胞(THP-1)中活性氧和肿瘤坏死因子-α形成的影响。研究了 LPS 联合 GYY4137 预处理后这些细胞中发生的转录组变化。我们获得的结果表明，Hsp70 和硫化氢供体可减少 LPS 作用激活的细胞中的炎症过程。Hsp70 和H ₂ S 供体的保护作用动力学不同，而硫化氢供体则更为有效。_{研究了内吞作用在H 2} S和Hsp70供体保护细胞免受LPS作用的机制中的作用。研究发现，GYY4137预处理LPS暴露的细胞可减少LPS诱导的各种促炎基因的诱导，并影响各种细胞内信号通路基因的表达。