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Evaluation of the biotransformation of alkali-silica reaction products by Alkalihalobacillus clausii and Bacillus thuringiensis
Cement and Concrete Composites ( IF 10.5 ) Pub Date : 2023-12-08 , DOI: 10.1016/j.cemconcomp.2023.105399
Domingos Jorge Ferreira da Silva , Kelly Cristina da Silva Brabes , Evaristo Alexandre Falcão

Currently, there are several ways to reduce the possibility of the occurrence of the alkali-silica reaction (ASR), when measures are adopted that attenuate the favorable conditions for its occurrence, avoiding possible damage to concrete structures. However, after ASR has been installed in the concrete structure, does not exist a completely efficient and economically viable solution that attenuate the expansive effects of ASR. In the present research, the ability of Alkalihalobacillus clausii (formerly Bacillus clausii) and Bacillus thuringiensis microorganisms to biotransform ASR products was studied, and thus contribute to an initial study and proposition of an alternative, to solve problems caused by expansions in concrete structures affected by ASR. The ASR products were synthesized under controlled laboratory conditions, from sources of amorphous (pyrex) silica and inoculated with microorganisms for a period of 40 days, being submitted to microstructural characterization using the XRD, DTG/TG and FTIR techniques in periods of 10, 20, 30 and 40 days. The results showed that the A. clausii and B. thuringiensis bacteria were able to biotransform the ASR products. XRD's showed that there was biotransformation of the elements contained in the samples due to the reduction in the degree of crystallinity of the samples inoculated with bacteria. Thermal analyzes showed that there was biotransformation of ASR products from reducing weight loss in samples inoculated with bacteria compared to non-inoculated samples. The FTIR's showed that the bacteria are capable of biotransforming the ASR products, reducing the vibration bands associated with the ASR products to values greater than 98 % of the initial product. The FTIR analyzes also showed that the diversity of elements present in the mineralogy of the aggregates and that are part of the metabolism of the bacteria can influence, depending on their availability, in the efficiency of the biotransformation process of the ASR products.



中文翻译:


克劳氏碱盐芽孢杆菌和苏云金芽孢杆菌对碱硅反应产物生物转化的评价



目前,有多种方法可以减少碱硅反应(ASR)发生的可能性,即采取措施削弱其发生的有利条件,避免对混凝土结构可能造成的损害。然而,在 ASR 安装在混凝土结构中后,并不存在完全有效且经济可行的解决方案来减弱 ASR 的膨胀效应。在本研究中,研究了克劳氏碱盐芽孢杆菌(以前的克劳氏芽孢杆菌)和苏云金芽孢杆菌微生物对 ASR 产品进行生物转化的能力,从而有助于初步研究和提出替代方案,以解决因混凝土结构膨胀而引起的问题。自动语音识别。 ASR 产品是在受控实验室条件下合成的,采用无定形(耐热玻璃)二氧化硅来源,并接种微生物 40 天,并在 10、20 天内使用 XRD、DTG/TG 和 FTIR 技术进行微观结构表征。 、 30 和 40 天。结果表明,克劳氏菌和苏云金芽孢杆菌能够对 ASR 产物进行生物转化。 XRD显示,由于接种细菌的样品结晶度降低,样品中所含元素发生了生物转化。热分析表明,与未接种的样品相比,接种细菌的样品中的 ASR 产品因减少重量损失而发生生物转化。 FTIR 显示细菌能够对 ASR 产品进行生物转化,将与 ASR 产品相关的振动带降低至初始产品的 98% 以上。 FTIR 分析还表明,聚集体矿物学中存在的元素的多样性以及细菌代谢的一部分可以影响 ASR 产品生物转化过程的效率,具体取决于其可用性。

更新日期:2023-12-08
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