Blood is the most commonly used body fluid for extracellular vesicle (EV) research. The composition of a blood sample and its derivatives (i.e., plasma and serum) are not only donor-dependent but also influenced by collection and preparation protocols. Since there are hundreds of pre-analytical protocols and over forty variables, the development of standard operating procedures for EV research is very challenging. To improve the reproducibility of blood EV research, the International Society for Extracellular Vesicles (ISEV) Blood EV Task Force proposes standardized reporting of (i) the applied blood collection and preparation protocol and (ii) the quality of the prepared plasma and serum samples. Gathering detailed information will provide insight into the performance of the protocols and more effectively identify potential confounders in the prepared plasma and serum samples. To collect this information, the ISEV Blood EV Task Force created the Minimal Information for Blood EV research (MIBlood-EV), a tool to record and report information about pre-analytical protocols used for plasma and serum preparation as well as assays used to assess the quality of these preparations. This tool does not require modifications of established local pre-analytical protocols and can be easily implemented to enhance existing databases thereby enabling evidence-based optimization of pre-analytical protocols through meta-analysis. Taken together, insight into the quality of prepared plasma and serum samples will (i) improve the quality of biobanks for EV research, (ii) guide the exchange of plasma and serum samples between biobanks and laboratories, (iii) facilitate inter-laboratory comparative EV studies, and (iv) improve the peer review process.

中文翻译：

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MIBlood-EV：用最少的信息提高血液细胞外囊泡研究的质量和可重复性

血液是细胞外囊泡 (EV) 研究最常用的体液。血液样本及其衍生物（即血浆和血清）的成分不仅取决于供体，而且还受到采集和制备方案的影响。由于有数百个预分析方案和四十多个变量，因此电动汽车研究标准操作程序的开发非常具有挑战性。为了提高血液 EV 研究的可重复性，国际细胞外囊泡协会 (ISEV) 血液 EV 工作组建议对 (i) 所应用的血液采集和制备方案以及 (ii) 制备的血浆和血清样本的质量进行标准化报告。收集详细信息将有助于深入了解方案的性能，并更有效地识别制备的血浆和血清样品中的潜在混杂因素。为了收集这些信息，ISEV 血液 EV 工作组创建了血液 EV 研究最小信息 (MIBlood-EV)，这是一种记录和报告有关用于血浆和血清制备的分析前方案以及用于评估的测定的信息的工具这些制剂的质量。该工具不需要修改已建立的本地预分析协议，并且可以轻松实施以增强现有数据库，从而通过荟萃分析实现基于证据的预分析协议优化。总而言之，深入了解制备的血浆和血清样本的质量将（i）提高 EV 研究生物库的质量，（ii）指导生物库和实验室之间血浆和血清样本的交换，（iii）促进实验室间比较EV 研究，以及 (iv) 改进同行评审流程。