The co-evolutionary struggle between plants and pathogens shapes vital defense mechanisms crucial for survival. AvrM14 selectively removes the 5′ cap from mRNA, disrupting key aspects of plant mRNA regulation, enhancing the flax rust fungus's infection capabilities. In contrast, quercetin, a plant flavonoid, serves as an antioxidant, stress reliever, and immune protector. This in silico study explores quercetin's inhibition of AvrM14, revealing high-affinity binding to the Nidux domain through molecular docking. Confirming stability in dynamic simulations, this pioneering research establishes quercetin as a functional AvrM14 inhibitor, suggesting its potential as a therapeutic agent against Melampsora lini's harmful impact on plant health.

植物和病原体之间的共同进化斗争塑造了对生存至关重要的重要防御机制。 AvrM14 选择性地去除 mRNA 的 5' 帽，破坏植物 mRNA 调节的关键方面，增强亚麻锈菌的感染能力。相比之下，槲皮素（一种植物类黄酮）可作为抗氧化剂、缓解压力和免疫保护剂。这项计算机研究探讨了槲皮素对 AvrM14 的抑制作用，揭示了通过分子对接与 Nidux 结构域的高亲和力结合。这项开创性的研究证实了动态模拟中的稳定性，将槲皮素确立为一种功能性 AvrM14 抑制剂，表明其作为治疗剂的潜力，可对抗 Melampsora lini's 有害菌对植物健康的影响。