The possible impact of ZnO and CuO nanoparticles (NPs) (individually and in binary mixture) was investigated using the freshwater microalgae, Scenedesmus obliquus. The present study shows the effect of nanoparticles on algae in OECD growth media, wastewater, and pond water during a 96-h toxicity test. At 0.1 mg/L concentration of the mixture of NPs, the reduction in the chlorophyll a content was 13.61 ± 1.34% (OECD media), 28.83 ± 1.85% (wastewater), and 31.81 ± 2.23% (pond water). Values of reduction in biomass were observed to be 42.13 ± 1.38, 39.96 ± 1.03, and 33.10 ± 1.29% for OECD media, wastewater, and pond water, respectively. The highest increase in lipid values was observed in the case of pond water (6.3 ± 1.31%). A significant increase in the value of EPS-generated protein was observed in the wastewater sample. EPS-generated carbohydrate values were increased in OECD media but decreased in the wastewater matrix. The transmission electron microscope images showed structural damage to algae cells due to the exposure to a mixture of nanoparticles at higher concentrations. Fourier transform infrared analysis showed an addition of bonds and differences in the peak and its intensity during exposure to high concentrations of NPs. Overall, this study gives fundamental insights into the interaction and toxicity of a mixture of NPs to algal species in different water matrices.

使用淡水微藻斜生栅藻研究了 ZnO 和 CuO 纳米颗粒 (NP)（单独和二元混合物）的可能影响。本研究展示了在 96 小时毒性测试中纳米颗粒对 OECD 生长介质、废水和池塘水中藻类的影响。当 NP 混合物浓度为 0.1 mg/L 时，叶绿素 a 含量的减少量为 13.61 ± 1.34%（OECD 介质）、28.83 ± 1.85%（废水）和 31.81 ± 2.23%（池塘水）。对于 OECD 介质、废水和池塘水，观察到生物量减少值分别为 42.13 ± 1.38、39.96 ± 1.03 和 33.10 ± 1.29%。池塘水的脂质值增幅最大 (6.3 ± 1.31%)。在废水样品中观察到 EPS 生成的蛋白质的价值显着增加。EPS 生成的碳水化合物值在 OECD 介质中增加，但在废水基质中降低。透射电子显微镜图像显示，由于暴露于较高浓度的纳米颗粒混合物，藻类细胞发生了结构损伤。傅里叶变换红外分析显示，在暴露于高浓度纳米颗粒期间，键增加，峰及其强度存在差异。总体而言，这项研究对不同水基质中纳米粒子混合物与藻类物种的相互作用和毒性提供了基本见解。