Liriomyza chinensis (Kato) is a formidable pest of Allium species, especially the Japanese bunching onion Allium fistulosum L. Recently, a novel biotype of L. chinensis (biotype B) has emerged, which causes more severe damage than the native biotype A. It has been reported that biotype B has frequently displaced biotype A in the Japanese bunching onion fields in Japan. As interbiotype hybridization is a possible factor that influences such displacement, interbiotype hybridization was conducted between L. chinensis biotypes A and B. Eggs were not laid under one-by-one crossing conditions; however, adult hybrid progeny of both sexes emerged from no-choice mating combinations―when multiple males and females were present. The fertility of F1 hybrid adults was also investigated, and backcrossed adults emerged from F1 females in both mating combinations. F1 males might have exhibited reproductive abnormalities because only a small number of backcross progeny emerged from the mating combinations using F1 males. Additionally, 3 representative endosymbionts (Wolbachia, Spiroplasma, and Cardinium) were investigated, and both biotypes were found to be infected by the same strain of Wolbachia. In addition, the courtship signals (tapping) of male adults differed between biotypes A and B as well as between F1 hybrids; the F1 males exhibited tapping behavior that was intermediate between biotypes A and B. Therefore, mating sounds serve as a form of premating reproductive isolation between biotypes A and B.

中文翻译：

---

美洲斑潜蝇生物型A和B之间的生物型间杂交（双翅目：农蝇科）

中华斑潜蝇 (Kato) 是葱属植物的一种可怕害虫，特别是日本斑葱 Allium fistulosum L。最近，出现了一种新的中华斑潜蝇生物型（生物型 B），其造成的危害比本地生物型 A 更为严重。据报道，在日本的日本洋葱田中，生物型 B 经常取代生物型 A。由于生物型间杂交是影响这种位移的可能因素，因此在中华羊草生物型A和B之间进行生物型杂交。在一对一杂交条件下不产卵；然而，当存在多个雄性和雌性时，两性的成年杂交后代是从无选择的交配组合中出现的。还研究了 F1 杂交成虫的生育力，回交成虫是从两种交配组合中的 F1 雌性中产生的。F1 雄性可能表现出生殖异常，因为使用 F1 雄性的交配组合中只有少量回交后代出现。此外，还对 3 种代表性的内共生体（沃尔巴克氏体、螺原体和卡迪氏菌）进行了研究，发现两种生物型都被同一株沃尔巴克氏体感染。此外，雄性成虫的求偶信号（轻拍）在生物型 A 和 B 之间以及 F1 杂交种之间也存在差异。F1 雄性表现出介于生物型 A 和 B 之间的敲击行为。因此，交配声音是生物型 A 和 B 之间交配前生殖隔离的一种形式。