Filamentous fd phages are rod-like viruses which can self-assemble into hierarchical structures including liquid crystals under predefined physiological conditions. In this study, Fe(III) counterion mediated condensation of fd viruses was investigated by Atomic force microscopy (AFM), Scanning electron microscopy (SEM) and Energy dispersive X-ray spectroscopy (EDX) analyses. Hierarchical supramolecular bundle-like structures obtained through Fe(III) treatment were used as biotemplates for iron oxide deposition process. Resulting phage-templated assemblies were collected with a magnet. Negative control samples without any viruses did not result in magnetic aggregate formation implying that the reaction was virus mediated. Morphology and elemental composition of magnet separated aggregates were investigated by SEM and EDX analyses. Such virus-templated bottom-up synthesized biomineralized materials could be further applied for interdisciplinary research areas including nanobioelectronics and biomedicine.

中文翻译：

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丝状FD病毒模板生物矿化超分子结构

丝状fd噬菌体是杆状病毒，可以在预定的生理条件下自组装成包括液晶在内的分层结构。在本研究中，通过原子力显微镜 (AFM)、扫描电子显微镜 (SEM) 和能量色散 X 射线光谱 (EDX) 分析研究了 Fe(III) 抗衡离子介导的 fd 病毒凝聚。通过 Fe(III) 处理获得的多级超分子束状结构被用作氧化铁沉积过程的生物模板。用磁铁收集所得的噬菌体模板组装体。不含任何病毒的阴性对照样品没有导致磁性聚集体形成，这意味着该反应是病毒介导的。通过 SEM 和 EDX 分析研究了磁力分离聚集体的形态和元素组成。这种以病毒为模板的自下而上合成的生物矿化材料可以进一步应用于包括纳米生物电子学和生物医学在内的跨学科研究领域。