Abstract

The influence of thermodynamic and kinetic conditions on the interaction of polyclonal antibodies to penicillins with the antibiotics of the penicillin group was studied in the system of a direct enzyme-linked immunosorbent assay (ELISA). Minimal differences in the cross reactions of the polyclonal antibodies with different penicillins were observed when ELISA was carried out at 4°C for 1 h. An increase in temperature and duration of the assay led to an increase in the reactivity of antibodies only to amoxicillin and significantly enhanced differences among the sensitivities of determination of individual penicillins. Under the chosen assay conditions, the following antibody cross-reactivities were obtained: penicillin G, 90%; ampicillin, 100%; and amoxicillin, 110%. The analytical sensitivity was 0.03 ng/mL for ampicillin, and the limit of ampicillin quantification in milk was 0.4 μg/L. The developed group-specific ELISA was used for the determination in milk of seven penicillins that are regulatorily controlled in foods and raw materials of animal origin: penicillin G, ampicillin, amoxicillin, cloxacillin, oxacillin, dicloxacillin and nafcillin.

中文翻译：

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免疫酶测定牛奶中青霉素的群体特异性对抗生素与多克隆抗体交叉反应温度和持续时间的依赖性

摘要

在直接酶联免疫吸附测定（ELISA）系统中研究了热力学和动力学条件对青霉素多克隆抗体与青霉素组抗生素相互作用的影响。ELISA 4℃ 1 h 时，多克隆抗体与不同青霉素的交叉反应差异极小。测定温度和持续时间的增加导致抗体仅对阿莫西林的反应性增加，并显着增强了单个青霉素测定灵敏度之间的差异。在选定的测定条件下，获得以下抗体交叉反应性：青霉素G，90％；氨苄西林，100%；和阿莫西林，110%。氨苄青霉素的分析灵敏度为0.03 ng/mL，牛奶中氨苄青霉素的定量限为0.4 μg/L。开发的组特异性 ELISA 用于测定牛奶中食品和动物源原材料中受监管控制的七种青霉素：青霉素 G、氨苄西林、阿莫西林、氯唑西林、苯唑西林、双氯西林和萘夫西林。