Abstract

The present study aims to investigate the possible liver and kidney toxicity mechanisms of prolonged acetamiprid (ACMP) induction and the protective effects of co-treatment with curcumin (Cur) on ACMP-induced liver and kidney complications. Forty male albino Wistar rats were divided into four groups (n = 10 in each). Group I (the control group) received 1% dimethyl sulfoxide, group II (the Cur-group) received Cur (100 mg/kg/day), group III (the ACMP-group) received acetamiprid (40 mg/kg/day), and group IV the (ACMP + Cur)-group received ACMP (40 mg/kg/day) plus Cur (100 mg/kg/day) for 30 days. Tissue samples from the liver and kidney were collected and prepared for biochemical, histological, and immunohistochemical analysis. Our results showed a significant increase in total oxidative stress levels and a significant decrease in the total antioxidant capacity in the liver and kidney tissue of the ACMP-group compared with those of the control group (p < 0.05 for all parameters). Also, the ACMP-group showed a disturbance in the structure of the liver and kidneys of rats compared to that of the control group. However, the (ACMP + Cur)-group showed significantly lower total oxidative stress levels and significantly higher levels of total antioxidant capacity than the ACMP-group, with histological similarity to the control group. Total oxidative stress and total antioxidant capacity could clarify the ACMP-induced hepatic and renal toxicity mechanisms that have been attenuated by Cur co-administration. These findings proposed that the co-administration of Cur with ACMP attenuated its toxicity by reducing oxidative stress and improving antioxidant capacity, indicating the role of Cur as an antioxidant in mitigating ACMP-toxicity.

中文翻译：

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姜黄素对啶虫脒引起的肝肾毒性保护作用的生化和组织学研究

摘要

本研究旨在探讨长期啶虫脒（ACMP）诱导可能的肝肾毒性机制，以及与姜黄素（Cur）联合治疗对ACMP诱发的肝肾并发症的保护作用。将 40 只雄性白化 ​​Wistar 大鼠分为四组（每组 n = 10）。I组（对照组）接受1%二甲亚砜，II组（Cur组）接受Cur（100 mg/kg/天），III组（ACMP组）接受啶虫脒（40 mg/kg/天） IV组(ACMP+Cur)-组接受ACMP(40mg/kg/天)加Cur(100mg/kg/天)，持续30天。收集并制备肝脏和肾脏的组织样本用于生化、组织学和免疫组织化学分析。我们的结果显示，与对照组相比，ACMP 组肝脏和肾脏组织的总氧化应激水平显着增加，总抗氧化能力显着下降（ 所有参数p < 0.05）。此外，与对照组相比，ACMP 组大鼠的肝脏和肾脏结构出现紊乱。然而，(ACMP + Cur)组显示出比ACMP组显着更低的总氧化应激水平和显着更高的总抗氧化能力水平，并且组织学与对照组相似。总氧化应激和总抗氧化能力可以阐明 ACMP 诱导的肝和肾毒性机制，这些机制已被 Cur 联合给药减弱。这些发现表明，Cur 与 ACMP 共同给药可通过减少氧化应激和提高抗氧化能力来减轻其毒性，表明 Cur 作为抗氧化剂在减轻 ACMP 毒性中的作用。