In this paper, the effects of resveratrol on the viability, morphology, biomechanics and bioelectricity of SH-SY5Y cells were studied by atomic force microscopy. MTT assay showed that resveratrol had a dose effect on SH-SY5Y cells, and its activity was related to drug concentration and drug action time. With the increase of resveratrol concentration or the extension of action time, the activity of SH-SY5Y cells decreased obviously. Atomic force microscope (AFM) was employed to quantitatively analyze the physical changes of cells. AFM study shows that resveratrol can transform SH-SY5Y cells from spindle to sphere, and increase the cell height and decrease the cell adhesion. Also, the elastic modulus increases under the action of low concentration of resveratrol decreases under the action of high concentration of resveratrol, and the electric signal decreases. This study reveals the impact of resveratrol on SH-SY5Y cells from the biological and biophysical perspectives, which is helpful for a more comprehensive understanding of the interaction mechanism between resveratrol and SH-SY5Y cells. These techniques have potential applications in evaluating the effects of chemical substances on cells and screening targeted drugs.

本文采用原子力显微镜研究了白藜芦醇对SH-SY5Y细胞活力、形态、生物力学和生物电的影响。MTT法显示白藜芦醇对SH-SY5Y细胞有剂量效应，其活性与药物浓度和药物作用时间有关。随着白藜芦醇浓度的增加或作用时间的延长，SH-SY5Y细胞的活性明显下降。采用原子力显微镜（AFM）定量分析细胞的物理变化。AFM研究表明，白藜芦醇可使SH-SY5Y细胞从纺锤形转变为球形，并增加细胞高度并降低细胞粘附。并且，在低浓度白藜芦醇作用下弹性模量增大，在高浓度白藜芦醇作用下弹性模量减小，电信号减小。本研究从生物学和生物物理学角度揭示了白藜芦醇对SH-SY5Y细胞的影响，有助于更全面地了解白藜芦醇与SH-SY5Y细胞之间的相互作用机制。这些技术在评估化学物质对细胞的影响和筛选靶向药物方面具有潜在的应用。