Surfactants were used to assist in the crystallization of lysozyme. Three types of surfactants with different structures (sodium dodecyl sulfate, cetyltrimethylammonium bromide, or betaine hydrochloride [BH]) were used. The study indicated that three surfactants were all capable of shortening the crystallization induction time of lysozyme, with BH inducing the shortest crystallization induction time (t = 1 min) and the largest crystal size (37 μm). The molecular interactions among each of the three surfactants aforementioned and lysozyme were also investigated by simulations. It was found that hydrogen bonding dominantly acted between the carboxyl structure of the surfactant BH and the lysozyme, which produced the most favorable conformation for the self-assembly of lysozyme (promote lysozyme orderly aggregation and shorten the crystallization induction time). On this basis, the mechanism of surfactant-promoted lysozyme crystallization was proposed.

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表面活性剂辅助溶菌酶结晶及其机理

使用表面活性剂来协助溶菌酶的结晶。使用三种不同结构的表面活性剂（十二烷基硫酸钠、十六烷基三甲基溴化铵或盐酸甜菜碱[BH]）。研究表明，三种表面活性剂均能缩短溶菌酶的结晶诱导时间，其中BH诱导的结晶诱导时间最短（t = 1 min），晶体尺寸最大（37 μm）。还通过模拟研究了上述三种表面活性剂与溶菌酶之间的分子相互作用。研究发现表面活性剂BH的羧基结构与溶菌酶之间主要以氢键作用，产生最有利于溶菌酶自组装的构象（促进溶菌酶有序聚集，缩短结晶诱导时间）。在此基础上，提出了表面活性剂促进溶菌酶结晶的机理。