Circular RNAs (circRNAs) can play essential roles in tumor development, including glioblastoma (GBM). The current study was performed to explore the function and mechanism of circ_0027446 in GBM progression. Circ_0027446, microRNA-346 (miR-346) and Phosphoglycerate kinase 1 (PGK1) levels were detected using reverse transcription-quantitative polymerase chain reaction assay. Cell behaviors were examined using Cell Counting Kit-8 assay, colony formation assay, EdU assay, flow cytometry, and transwell assay. Glycolytic metabolism was analyzed by commercial kits. The protein level was determined via western blot. The target interaction was analyzed by dual-luciferase reporter assay. Circ_0027446 function in vivo was explored by tumor xenograft assay. Circ_0027446 expression was significantly up-regulated in GBM samples and cells. Circ_0027446 down-regulation suppressed proliferation, invasion, glycolytic metabolism and enhanced apoptosis of GBM cells. MiR-346 was a target of circ_0027446, and circ_0027446 promoted GBM progression by sponging miR-346. PGK1 acted as a target gene of miR-346, and circ_0027446 interacted with miR-346 to regulate PGK1 expression. Overexpression of miR-346 inhibited malignant behaviors of GBM cells through down-regulating PGK1. Circ_0027446 contributed to tumor growth in vivo via miR-346/PGK1 axis. The current evidences demonstrated that circ_0027446 facilitated malignant progression of GBM through binding to miR-346 to up-regulate PGK1.

环状 RNA (circRNA) 在肿瘤发展中发挥重要作用，包括胶质母细胞瘤 (GBM)。本研究旨在探讨circ_0027446在GBM进展中的功能和机制。使用逆转录定量聚合酶链反应测定法检测 Circ_0027446、microRNA-346 (miR-346) 和磷酸甘油酸激酶 1 (PGK1) 水平。使用 Cell Counting Kit-8 测定、集落形成测定、EdU 测定、流式细胞术和 Transwell 测定检查细胞行为。通过商业试剂盒分析糖酵解代谢。通过蛋白质印迹测定蛋白质水平。通过双荧光素酶报告基因测定分析靶标相互作用。通过肿瘤异种移植测定探索了 Circ_0027446 的体内功能。GBM 样本和细胞中 Circ_0027446 表达显着上调。Circ_0027446 下调抑制 GBM 细胞的增殖、侵袭、糖酵解代谢并增强细胞凋亡。MiR-346 是 circ_0027446 的靶标，circ_0027446 通过海绵 miR-346 促进 GBM 进展。PGK1作为miR-346的靶基因，circ_0027446与miR-346相互作用来调节PGK1的表达。miR-346 的过表达通过下调 PGK1 抑制 GBM 细胞的恶性行为。Circ_0027446 通过 miR-346/PGK1 轴促进体内肿瘤生长。目前的证据表明，circ_0027446通过与miR-346结合上调PGK1，促进GBM的恶性进展。