Abstract

The mechanoelectrical feedback in the heart is based on the work of mechanically gated (MGCs) and mechanosensitive (MSCs) channels. Since microgravity alters the heart’s morphological and physiological properties, we hypothesized that the expression of both MGCs and MSCs would be affected. We employed RNA transcriptome sequencing to investigate changes in the gene transcript levels of MGCs and MSCs in isolated rat ventricular cardiomyocytes under control conditions and in a simulated microgravity environment. For the first time, our findings demonstrated that simulated microgravity induces alterations in the gene transcript levels of specific MGCs, such as TRPM7, TRPV2, TRPP1, TRPP2, Piezo1, TMEM63A, TMEM36B, and known MSCs, including K_2P2.1, K_2P3.1, Kir6.1, Kir6.2, Na_V1.5, Ca_V1.2, K_V7.1. However, other voltage-gated channels and channels lacking a voltage sensor remained unaffected. These findings suggest that the altered expression of MGCs and MSCs could lead to changes in the net currents across the membrane, ultimately impacting the heart’s function.

中文翻译：

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模拟微重力改变大鼠心室心肌细胞中机械门控和机械敏感离子通道基因转录的数量

摘要

心脏中的机电反馈基于机械门控 (MGC) 和机械敏感 (MSC) 通道的工作。由于微重力改变了心脏的形态和生理特性，我们假设 MGC 和 MSC 的表达都会受到影响。我们采用 RNA 转录组测序来研究在对照条件和模拟微重力环境下分离的大鼠心室心肌细胞中 MGC 和 MSC 基因转录水平的变化。我们的研究结果首次证明，模拟微重力会导致特定 MGC 的基因转录水平发生变化，例如 TRPM7、TRPV2、TRPP1、TRPP2、Piezo1、TMEM63A、TMEM36B 和已知的 MSC，包括 K 2P _2.1、K _2P 3.1 ，Kir6.1，Kir6.2，Na _V 1.5，Ca _V 1.2，K _V 7.1。然而，其他电压门控通道和缺少电压传感器的通道不受影响。这些发现表明，MGC 和 MSC 表达的改变可能导致跨膜净电流的变化，最终影响心脏功能。