Broad bean wilt virus 1 (BBWV-1, genus Fabavirus, family Secoviridae) infects many plants species, including important horticultural crops. Since some tobacco plants such as Nicotiana benthamiana, are susceptible to many viruses, they are used as experimental hosts and therefore abundant information about these species is available in databases. Here, the protein differential expression in N. benthamiana plants infected with BBWV-1 was studied using Liquid Chromatography coupled with Mass Spectrometry in tandem analysis (LC–MS/MS). Also, we studied the role of viral VP37 protein which is a BBWV-1 determinant of pathogenicity in the accumulation of the host proteins. For this purpose, we agroinfiltrated N. benthamiana plants with two BBWV-1 cDNA infectious clones: pBBWV1-Wt wilt type and the pBBWV1-G492C mutant knocking out for the viral VP37 protein. Virus infection induced the differential expression of 44 host proteins: 22 were overexpressed and the other 22 were underexpressed. These proteins were involved in important plant processes and located in different cell organelles, mainly in chloroplasts. Finally, 24 of these proteins were expressed differentially according to the presence of VP37 protein. Relation among host proteins that were differentially expressed, plant symptoms, and subcellular alterations are discussed.

蚕豆枯萎病毒 1（BBWV-1，蚕豆病毒属，Secoviridae科）感染许多植物物种，包括重要的园艺作物。由于一些烟草植物（例如本塞姆氏烟草）对许多病毒敏感，因此它们被用作实验宿主，因此数据库中提供了有关这些物种的丰富信息。在此，使用液相色谱与质谱串联分析 (LC-MS/MS) 研究了感染 BBWV-1 的本塞姆氏烟草植物中的蛋白质差异表达。此外，我们还研究了病毒 VP37 蛋白在宿主蛋白积累中的作用，VP37 蛋白是 BBWV-1 致病性的决定因素。为此，我们用两个 BBWV-1 cDNA 感染性克隆对本塞姆氏烟草植物进行农杆菌渗透：pBBWV1-Wt 枯萎型和敲除病毒 VP37 蛋白的 pBBWV1-G492C 突变体。病毒感染引起44种宿主蛋白​​的差异表达：22种过度表达，另外22种低表达。这些蛋白质参与重要的植物过程并位于不同的细胞器中，主要位于叶绿体中。最后，根据 VP37 蛋白的存在，其中 24 种蛋白出现差异表达。讨论了差异表达的宿主蛋白、植物症状和亚细胞改变之间的关系。