Abstract

Recently, lipid nanoparticles have been intensively studied as carriers of lipophilic drugs. In this work, we have studied the stability of nanoemulsions with paraffin oil, solid lipid nanoparticles with stearic acid, and nanostructured lipid particles with paraffin oil and stearic acid in a mass ratio of 1 : 1. The obtained results have shown that all studied lipid systems stabilized with nonionic surfactants Tween 60 and Span 60 were stable to aggregation and subsequent sedimentation for more than 30 days. The incorporation of lutein into the lipid particles has almost no effect on their stability, while the size of solid lipid nanoparticles and nanostructured lipid nanoparticles decreases from 28–30 to 15–17 nm. The bioavailability of lutein loaded in lipid nanoparticles is evaluated from their effect on the restoration of blood flow velocity by simulating hemic hypoxia. Almost immediately after the application of lipid nanoparticles, the blood flow velocity ceases to decrease, and a tendency to its restoration is observed in 5–10 min. This shows that lipid nanoparticles with paraffin oil and stearic acid are promising candidates for the delivery of lipophilic drugs.

中文翻译：

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用于叶黄素封装和递送的脂质纳米颗粒

摘要

近年来，脂质纳米颗粒作为亲脂性药物的载体得到了深入研究。在这项工作中，我们研究了石蜡油纳米乳液、硬脂酸固体脂质纳米颗粒以及石蜡油和硬脂酸质量比为 1:1 的纳米结构脂质颗粒的稳定性。所得结果表明，所有研究的脂质使用非离子表面活性剂 Tween 60 和 Span 60 稳定的系统可在 30 多天内稳定聚集和随后的沉降。将叶黄素掺入脂质颗粒中对其稳定性几乎没有影响，而固体脂质纳米颗粒和纳米结构脂质纳米颗粒的尺寸从28-30 nm减小到15-17 nm。通过模拟血液缺氧，通过脂质纳米颗粒负载叶黄素对血流速度恢复的影响来评估其生物利用度。在应用脂质纳米粒子后，血流速度几乎立即停止降低，并在 5-10 分钟内观察到恢复的趋势。这表明含有石蜡油和硬脂酸的脂质纳米颗粒是用于递送亲脂性药物的有希望的候选者。