A meta-analysis of literature data on essential amino acid (EAA) requirements of fish and crustaceans was performed to re-estimate EAA requirements and provide ideal amino acid profiles. Large numbers of studies have been conducted on EAA requirements of fish and crustaceans over the past decades. However, estimated EAA requirements of different species showed a large variation due to differences in methodological approaches and regression models. An extensive search and inclusion of literature on EAA requirements were conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines in this study, resulting in a dataset of 358 studies covering 77 species. The relative percentage of maximum weight gain was used as the outcome to evaluate the 10 EAA requirements. Forest plots analysis was employed for heterogeneity analysis, study weight allocation and re-estimation of the requirements. Results showed that trophic level, water temperature and dietary EAA inclusion levels affected EAA requirements estimation. The meta-analysis suggested that the estimated 10 EAA requirements (expressed as % crude protein, CP) of the fish were: arginine (Arg) 5.0 (±0.14), histidine (His) 2.0 (±0.11), isoleucine (Ile) 3.3 (±0.16), leucine (Leu) 4.9 (±0.24), valine (Val) 3.8 (±0.11), lysine (Lys) 5.2 (±0.12), sulfur amino acids (Met + Cys) 3.5 (±0.18), total aromatic amino acids (Phe + Tyr) 6.2 (±0.12), threonine (Thr) 3.5 (±0.18) and tryptophan (Trp) 0.9 (±0.08). Estimated EAA requirements (expressed as % CP) of crustaceans were Arg 5.1 (±0.31), His 2.5 (±0.15), Ile 4.3 (±0.97), Leu 5.7 (±0.08), Val 4.3 (±0.30), Lys 4.9 (±0.28), Met + Cys 3.2 (±0.18), Phe + Tyr 5.1 (±0.65), Thr 3.8 (±0.04) and Trp 0.8 (±0.15).

中文翻译：

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鱼类和甲壳类动物的必需氨基酸需求的荟萃分析


对鱼类和甲壳类必需氨基酸 (EAA) 需求的文献数据进行荟萃分析，以重新估计 EAA 需求并提供理想的氨基酸谱。在过去的几十年里，人们对鱼类和甲壳类动物的 EAA 需求进行了大量研究。然而，由于方法和回归模型的差异，不同物种的 EAA 需求估计值存在很大差异。本研究按照系统评价和荟萃分析指南的首选报告项目，对有关 EAA 要求的文献进行了广泛的搜索和纳入，形成了涵盖 77 个物种的 358 项研究的数据集。使用最大体重增加的相对百分比作为评估 10 EAA 要求的结果。森林图分析用于异质性分析、研究权重分配和需求的重新估计。结果表明，营养级、水温和膳食 EAA 含量水平影响 EAA 需求量的估计。荟萃分析表明，鱼类的 10 种 EAA 需求量（以粗蛋白百分比表示，CP）估计为：精氨酸 (Arg) 5.0 (±0.14)、组氨酸 (His) 2.0 (±0.11)、异亮氨酸 (Ile) 3.3 (±0.16)、亮氨酸 (Leu) 4.9 (±0.24)、缬氨酸 (Val) 3.8 (±0.11)、赖氨酸 (Lys) 5.2 (±0.12)、含硫氨基酸 (Met + Cys) 3.5 (±0.18)，总计芳香族氨基酸（Phe + Tyr）6.2（±0.12）、苏氨酸（Thr）3.5（±0.18）和色氨酸（Trp）0.9（±0.08）。甲壳类动物的估计 EAA 需求量（以 % CP 表示）为 Arg 5.1 (±0.31)、His 2.5 (±0.15)、Ile 4.3 (±0.97)、Leu 5.7 (±0.08)、Val 4.3 (±0.30)、Lys 4.9（ ±0.28)、Met + Cys 3.2 (±0.18)、Phe + Tyr 5.1 (±0.65)、Thr 3.8 (±0.04) 和 Trp 0.8 (±0.15)。