The growing societal concern regarding skin aging caused by ultraviolet (UV) exposure has spurred the quest for effective therapeutic solutions. Among these, secreted factors derived from adipose-derived stem cells (ADSC) have emerged as promising candidates with anti-photoaging properties. The secretome of ADSC stimulates resident fibroblasts, triggering antioxidant effects and promoting the synthesis of the extracellular matrix, thus counteracting the effects of photoaging. In this study, the potential of dried ADSC secretome (AD-Sc), obtained through a compressed fluid antisolvent process, is assessed as an anti-photoaging agent. In addition, liposome nanocarriers are used to encapsulate AD-Sc (Sc_Lipo) to enhance its delivery efficiency. The findings demonstrate that both AD-Sc and Sc_Lipo effectively reduce reactive oxygen species levels in UV-damaged human fibroblasts, upregulate the expression of HAS1 and COL1, and inhibit MMP2. Moreover, it is discovered that reverse electrodialysis (RED)-driven iontophoresis improves the intradermal delivery of Sc_Lipo, as validated in an ex vivo porcine skin. Finally, in an in vivo UV-induced wrinkling mice model, the Lipo+RED group shows noticeable skin photoaging improvements. This study provides additional evidence that a combined intradermal delivery system for AD-Sc may represent a viable therapeutic approach for mitigating and preventing photoaging in clinical settings.

中文翻译：

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通过脂质体和离子电渗皮内递送增强脂肪干细胞 (ADSC) 分泌组的抗光老化作用

社会对紫外线 (UV) 暴露引起的皮肤老化的日益关注，促使人们寻求有效的治疗解决方案。其中，源自脂肪干细胞（ADSC）的分泌因子已成为具有抗光老化特性的有前途的候选因子。 ADSC 的分泌组刺激常驻成纤维细胞，引发抗氧化作用并促进细胞外基质的合成，从而抵消光老化的影响。在这项研究中，评估了通过压缩流体反溶剂过程获得的干燥 ADSC 分泌组 (AD-Sc) 作为抗光老化剂的潜力。此外，脂质体纳米载体用于封装AD-Sc（Sc_Lipo）以提高其递送效率。研究结果表明，AD-Sc 和 Sc_Lipo 都能有效降低紫外线损伤的人成纤维细胞中的活性氧水平，上调 HAS1 和 COL1 的表达，并抑制 MMP2。此外，我们还发现，反向电渗析 (RED) 驱动的离子电渗疗法可改善 Sc_Lipo 的皮内递送，这在离体猪皮肤中得到了验证。最后，在体内紫外线诱导的皱纹小鼠模型中，Lipo+RED 组显示出明显的皮肤光老化改善。这项研究提供了额外的证据，表明 AD-Sc 的联合皮内给药系统可能代表了临床环境中减轻和预防光老化的可行治疗方法。