Tripartite ATP-independent periplasmic (TRAP) transporters are nutrient-uptake systems found in bacteria and archaea. These evolutionary divergent transporter systems couple a substrate-binding protein (SBP) to an elevator-type secondary transporter, which is a first-of-its-kind mechanism of transport. Here, we highlight breakthrough TRAP transporter structures and recent functional data that probe the mechanism of transport. Furthermore, we discuss recent structural and biophysical studies of the ion transporter superfamily (ITS) members and highlight mechanistic principles that are relevant for further exploration of the TRAP transporter system.

不依赖 ATP 的三联周质 (TRAP) 转运蛋白是细菌和古细菌中发现的营养吸收系统。这些进化趋异的转运蛋白系统将底物结合蛋白（SBP）与升降机型二级转运蛋白偶联，这是一种首创的转运机制。在这里，我们重点介绍突破性的 TRAP 转运蛋白结构和探索转运机制的最新功能数据。此外，我们讨论了离子转运蛋白超家族 (ITS) 成员的最新结构和生物物理研究，并重点介绍了与进一步探索 TRAP 转运蛋白系统相关的机制原理。