Colorectal cancer (CRC) presents a formidable challenge, characterized by a steadily increasing incidence. Current approaches to manage CRC, including chemotherapy and targeted therapies, are burdened with significant limitations such as resistance development, adverse events, and high costs. Hence, there is an urgent demand for a more promising alternative. Autocrine motility factor (AMF), known for its role in promoting cancer cell motility, exhibits a unique ability to selectively impede the growth of cancer cells. In our study, we have elucidated the specific inhibitory effect of AMF derived from DU145 prostate cancer cells (D-AMF) on the proliferation of CRC cells. D-AMF effectively downregulated the expression of glucose-6-phosphate dehydrogenase (G6PD) at both the mRNA and protein levels, resulting in a concurrent increase in the generation of reactive oxygen species (ROS). Notably, the combination of D-AMF and curcumin proved highly effective in eliminating curcumin-resistant CRC cells. Therefore, the use of D-AMF in conjunction with curcumin holds promise as an alternative treatment approach for CRC.

中文翻译：

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自分泌运动因子与姜黄素对结直肠癌细胞增殖的协同影响


结直肠癌（CRC）提出了严峻的挑战，其发病率稳步上升。目前治疗结直肠癌的方法，包括化疗和靶向治疗，都存在很大的局限性，例如耐药性的产生、不良事件和高成本。因此，迫切需要一种更有前景的替代方案。自分泌运动因子（AMF）以其促进癌细胞运动的作用而闻名，具有选择性阻碍癌细胞生长的独特能力。在我们的研究中，我们阐明了源自DU145前列腺癌细胞的AMF（D-AMF）对CRC细胞增殖的特异性抑制作用。 D-AMF 在 mRNA 和蛋白质水平上有效下调葡萄糖-6-磷酸脱氢酶 (G6PD) 的表达，导致活性氧 (ROS) 的产生同时增加。值得注意的是，D-AMF 和姜黄素的组合被证明在消除姜黄素耐药性 CRC 细胞方面非常有效。因此，D-AMF 与姜黄素联合使用有望成为 CRC 的替代治疗方法。