Developed countries have a high mortality rate from atherosclerosis and are frequently linked to inflammation and other blood lipid disorders. MicroRNA expression can affect atherosclerotic plaque formation, lipid metabolism, inflammation, and other related processes. The search aimed to determine whether microRNA-138-5p or microRNA-132-3p expression levels are related to patient atherogenic genes. Angiographic diagnostic method was used to select 45 healthy samples and 45 atherosclerosis patients, along with the laboratory and demographic information. After isolating peripheral blood mononuclear cells (PBMCs) from blood, the levels of miR-138 and miR-132 and the relative expression of Sirtuin 1 (SIRT1), signal transducer and activator of transcription-3 (STAT3), and CD36 genes measured using real-time PCR. miR-138 was upregulated compared to the control group in the atherosclerosis patient group (P < 0.05). In contrast, SIRT1 was downregulated in patients (P < 0.05). Our results also showed that the expression levels of miR-138 can use as a biomarker for atherosclerosis detection (P < 0.05). In addition, the expression of miR-138 with SIRT1 had a significant negative correlation (P < 0.05), and miR-132 was directly correlated with STAT3 (P < 0.01). Interestingly, STAT3 was negatively correlated with SIRT1 (P < 0.05) and positively with CD36 (P < 0.01). Since atherosclerosis has no specific clinical symptoms and early diagnosis is vital, the use of miR-138 diagnostic biomarkers can play an essential role in early diagnosis. Furthermore, this study highlights the overlap of SIRT1-STAT3-CD36 signaling pathways with miR-132 and miR-138 in atherosclerosis.

中文翻译：

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miR-138-5p、miR-132-3p、SIRT1、STAT3 和 CD36 与动脉粥样硬化患者血液单核细胞中动脉粥样硬化指数之间的关联


发达国家的动脉粥样硬化死亡率很高，并且经常与炎症和其他血脂紊乱有关。 MicroRNA的表达可以影响动脉粥样硬化斑块的形成、脂质代谢、炎症和其他相关过程。该研究旨在确定 microRNA-138-5p 或 microRNA-132-3p 表达水平是否与患者致动脉粥样硬化基因相关。采用血管造影诊断方法选择45名健康样本和45名动脉粥样硬化患者，以及实验室和人口统计信息。从血液中分离外周血单核细胞 (PBMC) 后，使用以下方法测量 miR-138 和 miR-132 的水平以及 Sirtuin 1 (SIRT1)、信号转导子和转录激活子 3 (STAT3) 以及 CD36 基因的相对表达实时荧光定量PCR。动脉粥样硬化患者组与对照组相比miR-138表达上调（P<0.05）。相反，SIRT1在患者中表达下调（P<0.05）。我们的结果还表明，miR-138的表达水平可以作为动脉粥样硬化检测的生物标志物（P<0.05）。此外，miR-138的表达与SIRT1呈显着负相关（P<0.05），miR-132与STAT3直接相关（P<0.01）。有趣的是，STAT3与SIRT1呈负相关（P<0.05），与CD36呈正相关（P<0.01）。由于动脉粥样硬化没有特定的临床症状，早期诊断至关重要，因此使用 miR-138 诊断生物标志物可以在早期诊断中发挥重要作用。此外，这项研究强调了 SIRT1-STAT3-CD36 信号通路与 miR-132 和 miR-138 在动脉粥样硬化中的重叠。