Despite a clear appreciation of the impact of human pathogens on community health, efforts to understand pathogen dynamics within populations often follow a narrow-targeted approach and rely on the deployment of specific molecular probes for quantitative detection or rely on clinical detection and reporting. Genomic analysis of wastewater samples for the broad detection of viruses, bacteria, fungi, and antibiotic resistance genes of interest/concern is inherently difficult, and while deep sequencing of wastewater provides a wealth of information, a robust and cooperative foundation is needed to support healthier communities. In addition to furthering the capacity of high-throughput sequencing wastewater-based epidemiology to detect human pathogens in an unbiased and agnostic manner, it is critical that collaborative networks among public health agencies, researchers, and community stakeholders be fostered to prepare communities for future public health emergencies or for the next pandemic. A more inclusive public health infrastructure must be built for better data reporting where there is a global human health risk burden. As wastewater platforms continue to be developed and refined, high-throughput sequencing of human pathogens in wastewater samples will emerge as a gold standard for understanding community health.

中文翻译：

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废水监测对呼吸道病毒、相关病原病毒和相关细菌抗生素抗性基因的功能范围是什么？

尽管人们清楚地认识到人类病原体对社区健康的影响，但了解人群内病原体动态的努力通常遵循狭窄目标的方法，并依赖于部署特定的分子探针进行定量检测或依赖于临床检测和报告。对废水样本进行基因组分析以广泛检测感兴趣/关注的病毒、细菌、真菌和抗生素抗性基因本质上是困难的，虽然废水的深度测序提供了丰富的信息，但需要一个强大的合作基础来支持健康社区。除了进一步提高基于废水的高通量测序流行病学以公正和不可知的方式检测人类病原体的能力外，还必须培养公共卫生机构、研究人员和社区利益相关者之间的合作网络，为社区为未来的公众做好准备。突发卫生事件或下一次大流行。必须建立更具包容性的公共卫生基础设施，以便在存在全球人类健康风险负担的情况下更好地报告数据。随着废水平台的不断开发和完善，废水样本中人类病原体的高通量测序将成为了解社区健康的黄金标准。