In this study, Neofusicoccum mediterraneum, a highly aggressive plant pathogen with a broad host range, including Eucalyptus spp., was investigated to determine its optimum medium and temperature for mycelial growth and chlamydospore production. The results revealed differences in the growth behaviour of N. mediterraneum under different in vitro conditions. Potato Sucrose Agar (PSA) was determined to be the most favourable environment for mycelial growth, achieving the maximum growth rate (GR) of 100% after 3 days of incubation at 25 °C. This was closely followed by cultures on Malt Extract Agar (MEA), exhibiting a GR of 100% after 4 days of incubation at 25 °C, while both substrates displayed optimal growth within the temperature range of 25 to 30 °C. The General Linear Model (GLM) unveiled significant differences in the growth rate across the different media (P = 0.000) and temperatures (P = 0.000). The interaction effect (media*temperature) retained its high significance throughout all incubation days (P < 0.0001). The binomial distribution and multinomial logistic regression analysis revealed that colony texture was significantly influenced by both the media (P = 0.022) and the temperature (P = 0.008). Additionally, temperature affected significantly the surface colour (P = 0.015) and reverse colour (P = 0.023) of the colony. Production of chlamydospores was observed only on PSA at 20 °C, 25 °C, 30 °C and 35 °C and on PDA at 25 °C and 30 °C. Both the medium and temperature had notable influences on the pathogen's growth, highlighting their importance in understanding its biology and behaviour. These findings contribute to the understanding of the phenotypic plasticity exhibited by N. mediterraneum towards temperatures and growth substrates.

在本研究中，地中海新孢子菌，一种高度侵袭性的植物病原体，宿主范围广泛，包括桉树 spp.进行了研究，以确定其菌丝生长和厚垣孢子产生的最佳培养基和温度。结果揭示了N 生长行为的差异。不同体外条件下的地中海。马铃薯蔗糖琼脂 (PSA) 被确定为菌丝生长最有利的环境，在 25 °C 培养 3 天后达到 100% 的最大生长率 (GR)。紧随其后的是麦芽提取物琼脂 (MEA) 培养物，在 25 °C 孵育 4 天后表现出 100% 的 GR，而两种基质在 25 至 30 °C 的温度范围内均表现出最佳生长。通用线性模型 (GLM) 揭示了不同介质 (P = 0.000) 和温度 (P = 0.000) 之间生长速率的显着差异。相互作用效应（介质*温度）在整个培养日中保持其高度显着性（P<<0.0001）。二项分布和多项Logistic回归分析表明，菌落质地受培养基（P = 0.022）和温度（P = 0.008）的显着影响。此外，温度显着影响菌落的表面颜色（P = 0.015）和反色（P = 0.023）。仅在 20°C、25°C、30°C 和 35°C 的 PSA 上以及在 25°C 和 30°C 的 PDA 上观察到厚垣孢子的产生。介质和温度对病原体的生长都有显着影响，凸显了它们在理解其生物学和行为方面的重要性。这些发现有助于理解N 所表现出的表型可塑性。地中海对温度和生长基质的影响。