Genus Klisinema (Desertifilaceae, Desertifilales) has only one species, Klisinema persicum, reported worldwide, and this species was found in soils around thermal springs (50°C) of high radiation regions. Simple filament cyanobacteria belonging to the genus Klisinema were collected from Korea, and cultured samples were studied through morphological, ecological and molecular analyses. Both Korean K. persicum and Klisinema koreana were found as epilithic species in freshwater gravel, which have different ecological characteristics from previously reported K. persicum. As a result of morphological analysis, keritomized cytoplasm was observed in K. koreana, and this feature was also newly observed in K. persicum that was discovered in Korea. In addition, K. koreana has a larger width/length ratio compared to K. persicum, and both species have an irregular thylakoid arrangement. Furthermore, as a result of phylogenetic analysis of 16S rRNA, the genus Klisinema formed a monophyletic lineage, and K. koreana strains were clearly separated from K. persicum strains to form a phylogenetic tree. Moreover, when comparing the secondary structure of the 16S–23S internal transcribed spacer region, K. koreana has a structural difference from K. persicum, especially in the structure of the Box-B helix. Therefore, Klisinema koreana sp. nov. was proposed as a new species belonging to the genus Klisinema of family Desertifilaceae.

中文翻译：

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朝鲜克利斯线虫 (Klisinema koreana sp.) 的研究十一月（沙漠丝科、蓝藻）来自韩国，基于形态学、生态学和分子分析

Klisinema属（Desertifilaceae，Desertifilales）在世界范围内只有一种，即Klisinema persicum，该种发现于高辐射地区温泉（50°C）周围的土壤中。从韩国收集了属于Klisinema属的单丝蓝藻，并通过形态学、生态学和分子分析对培养样品进行了研究。韩国K. persicum和Klisinema koreana都是在淡水砾石中发现的石生物种，其生态特征与之前报道的K. persicum不同。形态学分析的结果是，在K. koreana中观察到了细胞质的角质化，并且在韩国发现的K. persicum中也新观察到了这一特征。此外，与K. persicum相比， K. koreana具有更大的宽度/长度比，并且两个物种都具有不规则的类囊体排列。此外，16S rRNA的系统发育分析结果表明，Klisinema属形成了单系谱系，K. koreana品系与K. persicum品系明显分开，形成了系统发育树。此外，在比较16S-23S内转录间隔区的二级结构时，朝鲜堇菜与白堇菜有结构上的差异，特别是在Box-B螺旋的结构上。因此，Klisinema koreana sp。十一月被提议作为属于荒漠科Klisinema属的新种。