Neurodegenerative diseases impact the structure and operation of the nervous system, causing progressive and irreparable harm. Efforts for distinguishing neurodegenerative diseases in their early stages are continuing. Despite several biomarkers being identified, there is always search for more accurate and abundant ones. Additionally, it can be difficult to pinpoint the precise neurodegenerative disorder affecting a patient as the symptoms of these conditions frequently overlap. Numerous studies have shown that pathological changes occur years before clinical signs appear. Therefore, it is crucial to discover blood-based biomarkers for neurodegenerative diseases for easier and earlier diagnosis. Proximity extension assay is a unique proteomics method that uses antibodies linked to oligonucleotides for quantifying proteins with real-time PCR. Proximity extension assay can identify even low-quantity proteins using a small volume of specimens with increased sensitivity compared to conventional methods. In this article, we reviewed the employment of proximity extension assay technology to detect biomarkers or protein profiles for several neurodegenerative diseases.

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基于邻近延伸测定的神经退行性疾病和多发性硬化症的蛋白质组学研究

神经退行性疾病影响神经系统的结构和运作，造成渐进性和不可挽回的伤害。在早期阶段区分神经退行性疾病的努力仍在继续。尽管已经鉴定出多种生物标志物，但人们始终在寻找更准确、更丰富的生物标志物。此外，由于这些病症的症状经常重叠，因此很难精确定位影响患者的神经退行性疾病。大量研究表明，病理变化发生在临床症状出现前数年。因此，发现神经退行性疾病的血液生物标志物对于更容易和更早的诊断至关重要。邻近延伸测定是一种独特的蛋白质组学方法，它使用与寡核苷酸连接的抗体通过实时 PCR 来定量蛋白质。邻近延伸测定甚至可以使用少量样本来识别低含量的蛋白质，与传统方法相比，其灵敏度更高。在本文中，我们回顾了使用邻近延伸测定技术来检测几种神经退行性疾病的生物标志物或蛋白质谱。