Understanding the blood-feeding patterns of mosquitoes is essential for evaluating their potential as disease vectors, especially in urban areas where mosquitoes coexist with humans, domestic animals and wildlife. This study aimed to bridge a substantial gap in regional knowledge by identifying the blood meal sources of field-collected mosquitoes in domestic and open green environments from two urbanisations of temperate Argentina, the Área Metropolitana de Buenos Aires (AMBA) and Tandil, using molecular techniques. Female mosquitoes were collected from November 2019 to March 2020 and April–May 2021. A bipartite network analysis was performed for each environment and urbanisation. A total of 103 blood meals from Aedes (2 species) and Culex (7 species) were identified. Among these, five mammal and 18 bird species were recognised as hosts. Aedes mosquitoes exclusively fed on mammals, while Culex mosquitoes exhibited a broader host range including both birds and mammals. In AMBA, the open green environments were composed by more mosquito species than the domestic environments, while both presented similar numbers of vertebrate species. In contrast, in open green environments from Tandil only blood-fed Aedes albifasciatus were collected. For open green environments of AMBA and domestic environments of Tandil, results suggested some degree of host selection. For the three main vectors of diseases in the region, Aedes aegypti, Ae. albifasciatus and Culex pipiens molestus, we present the first molecular evidence of human blood meals in South America. Epidemiological significance of the present findings is discussed.

了解蚊子的吸血模式对于评估其作为疾病媒介的潜力至关重要，特别是在蚊子与人类、家畜和野生动物共存的城市地区。本研究旨在通过分子技术确定阿根廷温带两个城市——布宜诺斯艾利斯大都会区 (AMBA) 和坦迪尔的家庭和开放绿色环境中现场采集的蚊子的血粉来源，从而弥补区域知识的巨大差距。 。 2019 年 11 月至 2020 年 3 月以及 2021 年 4 月至 5 月收集雌性蚊子。对每个环境和城市化进行了双向网络分析。共鉴定出来自伊蚊（2 种）和库蚊（7 种）的 103 份血粉。其中，5种哺乳动物和18种鸟类被确认为宿主。伊蚊专门以哺乳动物为食，而库蚊则表现出更广泛的宿主范围，包括鸟类和哺乳动物。在 AMBA 中，开放式绿色环境中的蚊子种类比室内环境中的蚊子种类多，而两者的脊椎动物种类数量相似。相比之下，在坦迪尔的开放绿色环境中，仅收集了吸血的白带伊蚊。对于 AMBA 的开放绿色环境和坦迪尔的家庭环境，结果表明存在一定程度的宿主选择。该地区的三种主要疾病媒介为埃及伊蚊、伊蚊。 albifasciatus和Culex pipiensmolestus，我们首次提出了南美洲人类血粉的分子证据。讨论了当前研究结果的流行病学意义。