Abstract

The introduction of pollinator bumblebees (Bombus terrestris) or using the plant hormone, 2,4-dichlorophenoxyacetic acid (2,4-D), are common methods to improve tomato production in greenhouses. In this study, we compared the performance and transcriptomics of Solanum lycopersicum in response to B. terrestris and 2,4-D in greenhouse experiments. Tomato plants exposed to bumblebees had significantly higher fruiting rate and yield, weight and seed amounts, and significantly improved vitamin C, lycopene, soluble sugar contents, compared to hormone treatment. Bumblebee-treated tomatoes had 1171 significantly up-regulated genes, mainly enriched in photosynthesis, response to wounding, flavonoid biosynthesis, and carbohydrate biosynthetic process. By contrast, a total of 718 genes of 2,4-D-treated tomatoes were significantly up-regulated, which were mainly enriched in UDP-glucosyltransferase activity, response to chemical or auxin. Quantitative real-time PCR analysis of 14 candidate genes all exhibited good reproduction with the transcriptome data. These responses implied that bumblebee-treated tomatoes had improved photosynthesis and carbon-fixation capacity compared to 2,4-D-treated tomatoes. The use of bumblebees, as natural pollinators should be widely adopted in greenhouse horticulture to increase tomato yields and its nutritional contents.

中文翻译：

---

番茄（Solanum lycopersicum）对熊蜂（膜翅目、蜜蜂科）和 2,4-二氯苯氧乙酸的反应比较性能和转录组学

摘要

引入授粉熊蜂 (Bombus terrestris) 或使用植物激素 2,4-二氯苯氧基乙酸 (2,4-D ），是提高温室番茄产量的常用方法。在本研究中，我们比较了 Solanum lycopersicum 响应 B 的性能和转录组学。 terrestris 和 2,4-D 在温室实验中的应用。与激素处理相比，暴露于熊蜂处理的番茄植株的结果率和产量、重量和种子量显着提高，维生素C、番茄红素、可溶性糖含量显着提高。经熊蜂处理的番茄有 1171 个显着上调的基因，主要富集在光合作用、受伤反应、类黄酮生物合成和碳水化合物生物合成过程中。相比之下，2,4-D处理的番茄共有718个基因显着上调，主要富集UDP-葡萄糖基转移酶活性、对化学物质或生长素的反应。 14个候选基因的实时定量PCR分析与转录组数据均表现出良好的重现性。这些反应表明，与 2,4-D 处理的番茄相比，经大黄蜂处理的番茄具有改善的光合作用和固碳能力。温室园艺应广泛采用熊蜂作为天然传粉媒介，以提高番茄产量和营养成分。