During field surveys conducted in 2021 in western Jordanian heights, phytoplasma-like symptoms were observed, including leaf reddening along with slow decline in pear (pear decline symptoms) and leaf yellowing followed by scorch in apple trees. Abundant populations of the psyllid Cacopsylla bidens were found in the pear trees. Nested PCR amplifying 16S rDNA detected phytoplasmas in 63% and 36% of symptomatic pear and apple samples, respectively, and in 46% of pooled C. bidens specimens. No phytoplasmas were detected in symptomless plant samples. PCR product nucleotide sequence analyses attributed the phytoplasmas detected in pear to ‘Candidatus Phytoplasma pyri’, ‘Ca. P. solani’, ‘Ca. P. omanense’, and ‘Ca. P. aurantifolia’, and those identified in apple to ‘Ca. P. solani’ and ‘Ca. P. omanense’. All phytoplasma strains identified in C. bidens were attributed to ‘Ca. P. pyri’. To the best of our knowledge, this is the first report of phytoplasmas associated with pear and apple diseases, including pear decline, in Jordan. Even though a limited number of pome fruit samples were analyzed, a surprising diversity was found among detected phytoplasmas. Further studies will be carried out to investigate the complex etiology of such diseases, and the ‘Ca. P. pyri’ vectoring activity of C. bidens.

2021 年在约旦西部高地进行的实地调查期间，观察到了类似植原体的症状，包括梨树叶子变红并缓慢衰退（梨衰退症状），以及苹果树叶子变黄然后烧焦。在梨树中发现了大量的木虱Cacopsylla bidens。巢式 PCR 扩增 16S rDNA 分别在 63% 和 36% 的有症状梨和苹果样本中检测到植原体，在 46% 的混合样本中检测到植原体。鬼针草标本。在无症状的植物样品中未检测到植原体。 PCR产物核苷酸序列分析将梨中检测到的植原体归因于“CandidatusPhytoplasmapyri”、“Ca。C 的引导活性。拜登”。 P.pyri' Ca P.pyri”。据我们所知，这是约旦首次报道与梨和苹果病害（包括梨衰退）相关的植原体。尽管分析了有限数量的仁果样品，但在检测到的植原体中发现了令人惊讶的多样性。我们将开展进一步的研究来调查此类疾病的复杂病因和“Ca. 归因于“C. 中鉴定的所有植原体菌株。 bidens。 P. omanense’。 Ca。 P. solani”和“Ca。 P. aurantifolia”，以及在苹果中鉴定出的那些“Ca。 P. omanense”和“Ca。 P. solani'，'