This study reports the determination of Tinidazole (TDL) using a modified glassy carbon electrode, poly(bis(2,2′-bipyridine)diresorcinateruthenium(III) chloride) (poly(BBPDRRuC)/GCE) by a newly synthesized mixed ligand complex, bis-(2,2′-bipyridine)diresorcinateruthenium(III) chloride(BBPDRRuC). Electrochemical impedance spectroscopy (EIS) and cyclic voltamettry (CV) results demonstrated modification of the surface of the electrode by a conductive, and electroactive polymer film leading to an enhanced effective electrode surface area and their electrocatalytic role. Appearance of an irreversible reductive peak at much reduced potential with four folds current enhancement at poly(BBPDRRuC)/GCE showed the catalytic effect of the modifier by reduction of TDL. Square wave voltammetry current response of poly(BBPDRRuC)/GCE showed linear dependence on concentration of TDL in the range 10˗ 3.0 × 10 M with LoD and LoQ of 2.5 nM, and 8.2 nM, respectively. The TDL level in the studied tablet brands were in the range 96.6–101.1% of their labeled values. Spike recovery results in tablet, and human blood serum samples were in the range 98.3˗100.4%, and 98.85 ˗ 99.89%, respectively, and interference recovery results with <4.5% error. The developed method required a simple electrode modification step, a relatively chip, an easily available and non-toxic modifier, provides the least LoD, and reasonably wider linear dynamic range, and had excellent performance for the determination of TDL in tablet formulation and serum samples as compared with recently reported voltammetric methods.

中文翻译：

---

使用聚(双(2,2′-联吡啶)二间苯二酚氯化钌(III)氯化物)修饰玻碳电极选择性方波伏安法测定药物制剂和人尿样中的替硝唑

本研究报告了使用改性玻碳电极、聚(双(2,2′-联吡啶)二间苯二甲酸钌(III)氯化物)(聚(BBPDRRuC)/GCE)和新合成的混合配体复合物测定替硝唑(TDL)，双-(2,2′-联吡啶)二间苯二甲酸钌(III)氯化物(BBPDRRuC)。电化学阻抗谱 (EIS) 和循环伏安法 (CV) 结果表明，导电和电活性聚合物薄膜对电极表面进行了改性，从而增强了有效电极表面积及其电催化作用。在聚(BBPDRRuC)/GCE 处，在大大降低的电位下出现不可逆还原峰，电流增强四倍，这表明改性剂通过还原 TDL 发挥催化作用。 Poly(BBPDRRuC)/GCE 的方波伏安法电流响应在 10˗ 3.0 × 10 M 范围内与 TDL 浓度呈线性相关，LoD 和 LoQ 分别为 2.5 nM 和 8.2 nM。所研究的片剂品牌的 TDL 水平在其标签值的 96.6-101.1% 范围内。片剂和人血清样品中的加标回收率结果分别在 98.3˗100.4% 和 98.85˗99.89% 范围内，干扰回收率结果误差 <4.5%。所开发的方法需要简单的电极修饰步骤、相对的芯片、易于获得且无毒的修饰剂，提供最小的LoD和相当宽的线性动态范围，并且对于片剂制剂和血清样品中TDL的测定具有优异的性能与最近报道的伏安法相比。