Basal stem rot (BSR) is a detrimental disease in oil palm (Elaeis guineensis) due to the host's lack of symptoms during the early phase of infection. Early detection of BSR at non-symptomatic phase is feasible via molecular-based approaches and has become the major interest among researchers. Our previous study had identified a differentially expressed gene encoding auxin efflux carrier component 5-like (EgPIN5) at 11 day-post-inculation of Ganoderma boninense (symptomless early stage of infection) on oil palm seedlings. We further evaluated the gene expression on samples collected from an oil palm plantation at different BSR stages (symptomless, mild and severe). Our findings revealed that EgPIN5 was expressed in symptomless group but was not expressed in either mild or severe BSR groups. Next, we developed a sandwich-type aptamer-based lateral flow assay (LFA) against EgPIN5 RNA by adapting the versatile DNA-functionalized gold nanoparticles for colorimetric detection. The LFA strip demonstrated visible colorimetric signal of EgPIN5 RNA at 12.5 ng/μL with limit of detection at 2.45 ng/μL using a scanning device. Based on our consistent results, we presented EgPIN5 as a diagnostic biomarker for symptomless BSR infection in oil palm. Utilization of LFA assay strip for practical EgPIN5 RNA detection is hoped to encourage establishment of a fast, cheap, and sensitive point-of-care detection system in plant biology.

基部茎腐病（BSR）是油棕（Elaeis guineensis）的一种有害疾病，因为宿主在感染早期阶段没有症状。通过基于分子的方法在非症状阶段早期检测 BSR 是可行的，并且已成为研究人员的主要兴趣。我们之前的研究在油棕幼苗上接种博宁灵芝（无症状感染早期）11天后发现了编码生长素外流载体成分5样（EgPIN5 ）的差异表达基因。我们进一步评估了从油棕种植园采集的不同 BSR 阶段（无症状、轻度和严重）样本的基因表达。我们的研究结果显示，EgPIN5在无症状组中表达，但在轻度或重度 BSR 组中均不表达。接下来，我们通过采用多功能 DNA 功能化金纳米颗粒进行比色检测，开发了一种针对EgPIN5 RNA的基于夹心型适体的侧流测定 (LFA) 。LFA 试纸条显示EgPIN5 RNA 的可见比色信号为 12.5 ng/μL，使用扫描装置的检测限为 2.45 ng/μL。基于我们一致的结果，我们提出EgPIN5作为油棕无症状 BSR 感染的诊断生物标志物。利用 LFA 检测条进行实用的EgPIN5 RNA 检测有望鼓励在植物生物学中建立快速、廉价且灵敏的即时检测系统。