Ticks are obligate ectoparasites and vectors of pathogens affecting health, agriculture, and animal welfare. This study collected ticks from the cattle and questing ticks of 24 Magdalena Medio Antioquia region cattle farms. Genomic DNA was extracted from the specimens (individual or pools) of the 2088 adult ticks collected from cattle and 4667 immature questing ticks collected from pastures. The molecular detection of Babesia, Anaplasma, Coxiella and Rickettsia genera was performed by polymerase chain reaction amplification and subsequent DNA sequencing. In a total of 6755 Rhipicephalus microplus DNA samples, Anaplasma marginale was the most detected with a frequency of 2% (Confidence Interval- CI 1.68–2.36), followed by Babesia bigemina with 0.28% (CI 0.16–0.44), Coxiella spp. with 0.15% (CI 0.07–0.27), and Rickettsia spp. with 0.13% (CI 0.06–0.25). Molecular analysis of the DNA sequences obtained from the tick samples revealed the presence of Coxiella-like endosymbiont and R. felis. These results demonstrated the diversity of microorganisms present in R. microplus ticks predominantly associated with cattle and questing ticks from livestock agroecosystems, suggesting their role as reservoirs and potential biological vectors of these microorganisms on the studied sites. Also, it emphasizes the need to combine acarological surveillance with clinical diagnoses and control strategies on regional and national levels.

蜱是影响健康、农业和动物福利的专性体外寄生虫和病原体载体。本研究收集了马格达莱纳·梅迪奥·安蒂奥基亚地区 24 个养牛场的牛蜱和探索蜱。从从牛身上收集的 2088 只成年蜱和从牧场收集的 4667 只未成熟蜱的样本（个体或池）中提取基因组 DNA。通过聚合酶链式反应扩增和随后的DNA测序对巴贝虫属、无形体属、柯克斯体属和立克次体属进行分子检测。在总共 6755 个微小扇头扇贝DNA 样本中，边缘无形体检测到的频率最多，为 2%（置信区间 - CI 1.68-2.36），其次是巴贝虫，为 0.28%（CI 0.16-0.44），柯克斯体属。0.15% (CI 0.07–0.27) 和立克次体属。0.13% (CI 0.06–0.25)。对从蜱样本中获得的 DNA 序列进行的分子分析揭示了类柯克斯体内共生体和猫立克体的存在。这些结果证明了R. microplus蜱中存在的微生物多样性，这些蜱主要与牛和来自牲畜农业生态系统的探寻蜱有关，表明它们在研究地点作为这些微生物的储存库和潜在生物载体的作用。此外，它还强调需要将螨学监测与区域和国家层面的临床诊断和控制策略相结合。