NADPH-cytochrome P450 reductase (CPR) is a key enzyme transferring electrons to cytochrome P450. In tomatoes (Solanum lycopersicum), two CPR genes, SlCPR1 and SlCPR2, were identified. In all the tested tomato tissues, SlCPR2 showed higher expression levels than SlCPR1. SlCPR2 expression increased significantly with jasmonic acid treatment. No significant changes were observed with salicylic acid or drought stress treatment. The cDNA of SlCPRs were expressed in Escherichia coli without any amino acid modification. And the heterologously expressed SlCPR enzymes were reacted with several protein and chemical substrates. SlCPR2 was more active than SlCPR1. Both SlCPR1 and SlCPR2 exhibited strong activity across a pH range of 6.0 to 9.0, with peak activity at pH 8.0. The study opens possibilities for CPR control, biocatalyst development, and exploring oxidase enzyme functions.

中文翻译：

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大肠杆菌中的异源表达和番茄 CPR 酶的功能表征

NADPH-细胞色素 P450 还原酶 (CPR) 是将电子转移到细胞色素 P450 的关键酶。在番茄 (Solanum lycopersicum) 中，鉴定出两个 CPR 基因：SlCPR1 和 SlCPR2。在所有测试的番茄组织中，SlCPR2 的表达水平均高于 SlCPR1。茉莉酸处理后 SlCPR2 表达显着增加。水杨酸或干旱胁迫处理没有观察到显着变化。 SlCPRs的cDNA在大肠杆菌中表达，没有任何氨基酸修饰。异源表达的SlCPR酶与多种蛋白质和化学底物发生反应。 SlCPR2 比 SlCPR1 更活跃。 SlCPR1 和 SlCPR2 在 pH 6.0 至 9.0 范围内均表现出很强的活性，在 pH 8.0 时达到峰值活性。该研究为心肺复苏控制、生物催化剂开发和探索氧化酶功能开辟了可能性。