Growth performance, which can be evaluated using various body measurements, is crucial for providing red meat for human consumption. This study aims to improve the growth performance of sheep by identifying the different genotypes of leptin and IGFI genes associated with higher body measurements. In this study, we collected blood samples from Barki (n = 30) and Farafra (n = 30) sheep, and measured their body height, width, length, and weight, and tail width and length to assess their growth performance. We used single strand conformation polymorphism polymerase chain reaction (SSCP-PCR) and sequencing analysis to identify single nucleotide polymorphisms (SNPs) and different genotypes of the leptin and insulin-like growth factor receptor (IGFI) genes associated with sheep body measurements. The leptin gene (209 bp) and IGFR gene (345 bp) were found to have three (TT, TG, and GG) and (TT, TC, and CC) genotypes, respectively, by SSCP-PCR. Sequence analysis revealed a SNP in the leptin gene (200G > T) with two alleles (G allele: OR058867 and T allele: OR058868). This SNP resulted in the substitution of the amino acid valine with leucine, which increased the width, length, and weight of the body and tail width in the heterozygous form (TG). The IGFI gene had three SNPs: 305 G > T in TT genotype (OR260670), 184C > T, 265T > C, and 305G > T in TC genotype (OR260669), and 265T > C in CC genotype (OR260671). The TC genotype of the IGFR gene was correlated with higher values of body measurements. Barki sheep were found to have the highest values for body weight, width, length, and tail width compared to Farafra sheep. We recommend the use of entire Barki sheep in inbreeding programs to improve growth performance in Egyptian sheep.

中文翻译：

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巴基羊和法拉弗拉羊瘦素和IGFI基因多态性与体型测量的相关性分析

生长性能可以通过各种身体测量来评估，对于提供供人类食用的红肉至关重要。本研究旨在通过鉴定与较高体型测量相关的瘦素和 IGFI 基因的不同基因型来提高绵羊的生长性能。在本研究中，我们采集了Barki（n = 30）和Farafra（n = 30）羊的血液样本，测量了它们的体高、宽度、长度和重量以及尾巴宽度和长度，以评估它们的生长性能。我们使用单链构象多态性聚合酶链反应（SSCP-PCR）和测序分析来鉴定与羊体测量相关的瘦素和胰岛素样生长因子受体（IGFI）基因的单核苷酸多态性（SNP）和不同基因型。通过SSCP-PCR发现瘦素基因(209bp)和IGFR基因(345bp)分别具有三种(TT、TG和GG)和(TT、TC和CC)基因型。序列分析揭示瘦素基因中的SNP(200G  T)具有两个等位基因(G等位基因：OR058867和T等位基因：OR058868)。该 SNP 导致氨基酸缬氨酸被亮氨酸取代，从而增加了杂合形式 (TG) 的身体宽度、长度和重量以及尾部宽度。 IGFI基因具有3个SNP：305G ＞305。 TT基因型(OR260670)中的T，184C > T，265T > C、305G > TC基因型(OR260669)中的T，以及265T > CC 基因型中的 C (OR260671)。 IGFR 基因的 TC 基因型与较高的身体测量值相关。与法拉弗拉羊相比，巴基羊的体重、宽度、长度和尾宽值最高。我们建议在近亲繁殖计划中使用整只巴基羊，以提高埃及羊的生长性能。