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Sexual behavior and chemical signaling in Opsiphanes cassina (Lepidoptera: Nymphalidae)
Chemoecology ( IF 1.8 ) Pub Date : 2023-12-20 , DOI: 10.1007/s00049-023-00393-z
Jenifer J. Bustos-Cortés , Rosa C. Aldana-De la Torre , Anuar Morales-Rodríguez , Carolina Chegwin-Angarita , Alicia Romero-Frías

Behavior modification using chemical stimuli is a promising tactic for integrated management of lepidopteran pests. Therefore, this approach was evaluated for the management of the butterfly Opsiphanes cassina Felder 1862, a defoliating pest in oil palm plantations. To achieve this aim, we observed adults’ sexual behavior under laboratory conditions and identified glandular structures in the abdomen and hind wings of males that are associated with the production of sexual pheromones in taxonomically related insects. It was also determined that male calls to females occur at 18:00 h when they fill their glandular structures, tremble, and make abdominal movements while possibly releasing pheromones. The hairpencils of the hind wings are also exposed during this time. Furthermore, the analysis using Gas Chromatography-Mass Spectrometry (GC–MS) on the samples obtained by Solvent Assisted Extraction (SAE) revealed the identification of three male-specific volatile organic compounds (VOCs), including (E)-nerolidol, vanillin, and (Z)-7-heptadecene. The Electroantennographic (EAG) evaluation demonstrated that females respond to the compounds, indicating their likely involvement in intraspecific interactions of the species. However, the behavioral evaluation did not confirm their attractive effect under the conditions employed. Nevertheless, the identified male-specific semiochemicals could be an alternative for inclusion in integrated pest management (IPM) of this pest in oil palm crops. Therefore, it is recommended to evaluate them under field conditions.



中文翻译:

Opsiphanes cassina 的性行为和化学信号(鳞翅目:蛱蝶科)

使用化学刺激来改变行为是鳞翅目害虫综合治理的一种很有前途的策略。因此,我们对这种方法对蝴蝶Opsiphanes cassina Felder 1862(一种油棕种植园的落叶害虫)的管理进行了评估。为了实现这一目标,我们在实验室条件下观察了成虫的性行为,并确定了雄性腹部和后翅的腺体结构,这些腺体结构与分类学相关昆虫中性信息素的产生有关。研究还确定,雄性对雌性的叫声发生在 18:00,此时雌性的腺体结构充满,颤抖,腹部运动,同时可能释放信息素。此时后翅的毛笔也暴露出来。此外,使用气相色谱-质谱法(GC-MS)对溶剂辅助萃取(SAE)获得的样品进行分析,结果发现三种男性特有的挥发性有机化合物(VOC),包括(E)-橙花叔醇、香草醛和(Z)-7-十七碳烯。触角电图(EAG)评估表明雌性对这些化合物有反应,表明它们可能参与了物种内的相互作用。然而,行为评估并未证实它们在所采用的条件下的吸引力效果。尽管如此,已确定的雄性特异性化学信息素可以作为纳入油棕作物害虫综合害虫管理(IPM)的替代方案。因此,建议在现场条件下对其进行评估。

更新日期:2023-12-20
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