The placenta plays a pivotal role in the maintenance of normal pregnancy, but how it forms, matures, and performs its function remains poorly understood. Here, we describe a novel mouse line (Prl3d1-iCre) that expresses iCre recombinase under the control of the endogenous prl3d1 promoter. Prl3d1 has been proposed as a marker for distinguishing trophoblast giant cells (TGCs) from other trophoblast cells in the placenta. The in vivo efficiency and specificity of the Cre line were analyzed by interbreeding Prl3d1-iCre mice with B6-G/R reporter mice. Through anatomical studies of the placenta and other tissues of Prl3d1-iCre/+; B6-G/R mouse mice, we found that the tdTomato signal was expressed in parietal trophoblast giant cells (P-TGCs). Thus, we report a mouse line with ectopic Cre expression in P-TGCs, which provides a valuable tool for studying human pathological pregnancies caused by implantation failure or abnormal trophoblast secretion due to aberrant gene regulation.

中文翻译：

---

Prl3d1-Cre小鼠系选择性诱导壁层滋养层巨细胞Cre重组酶的表达

胎盘在维持正常妊娠方面发挥着关键作用，但人们对它如何形成、成熟和发挥其功能仍知之甚少。在这里，我们描述了一种新型小鼠品系（Prl3d1-iCre），它在内源性prl3d1启动子的控制下表达 iCre 重组酶。Prl3d1 已被提议作为区分胎盘中滋养层巨细胞 (TGC) 与其他滋养层细胞的标记物。通过将 Prl3d1-iCre 小鼠与 B6-G/R 报告小鼠杂交来分析 Cre 系的体内效率和特异性。通过对Prl3d1-iCre/+胎盘和其他组织的解剖学研究；B6-G/R 小鼠中，我们发现 tdTomato 信号在壁层滋养层巨细胞 (P-TGC) 中表达。因此，我们报道了 P-TGC 中异位 Cre 表达的小鼠系，这为研究由于基因调控异常而引起的着床失败或滋养层分泌异常引起的人类病理妊娠提供了有价值的工具。