The mobilization step in a two-step capillary isoelectric focusing protocol is discussed by means of dynamic computer simulation data for systems without and with spacer compounds that establish their zones at the beginning and end of the focusing column. After focusing in an electroosmosis-free environment (first step), mobilization (second step) can be induced electrophoretically, by the application of a hydrodynamic flow, or by a combination of both means. Dynamic simulations provide insight into the complexity of the various modes of electrophoretic mobilization and dispersion associated with hydrodynamic mobilization. The data are discussed together with the relevant literature.

中文翻译：

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两步毛细管等电聚焦中的移动：通过计算机模拟评估概念

通过动态计算机模拟数据讨论了两步毛细管等电聚焦方案中的动员步骤，该数据适用于没有和有间隔化合物的系统，这些间隔化合物在聚焦柱的开始和结束处建立了它们的区域。在无电渗环境中聚焦后（第一步），可以通过应用流体动力流或通过两种方式的组合以电泳方式诱导动员（第二步）。动态模拟可以深入了解与流体动力流动相关的各种电泳流动和分散模式的复杂性。这些数据与相关文献一起讨论。