The addition of exogenous endocrine disrupting compounds (EDCs) like estrone, in the food chain through the aquatic system, disrupts steroid biosynthesis and metabolism by altering either the genomic or non-genomic pathway that eventually results in various diseases. Thus, bioremediation of these compounds is urgently required to prevent their addition and persistence in the environment. Enzymatic degradation has proven to be a knight in shining armour as it is safe and generates no toxic products. The multicopper oxidases (E.C. 1.10.3.2 benzenediol: oxygen oxidoreductase), laccase with the potential to degrade both phenolic and non-phenolic substrates has recently gained attention. In this study, the laccase was purified, characterized, and used to study estrone degradation. The culture filtrate (crude laccase) was concentrated and precipitated using cold-acetone and dialyzed against tris buffer (50 mM) giving a four-fold partially purified form, with 45.56% yield and 204.14 U/mg as specific activity and a single peak at 250–300 nm. The partially purified laccase was approximately 80 kDa as estimated by SDS-PAGE preferred ABTS as substrate. Both crude and partially purified laccase showed maximum activity at pH 3.0, 40 °C, and 4 mM ABTS. Kinetic constants (Km, Vmax) of crude and partially purified laccase were found to be 0.83 mM; 494.31 mM/min, and 0.58 mM; 480.54 mM/min respectively. Iron sulphate and sodium azide inhibited laccase maximally. Crude and partially purified laccase degradation efficiency was 87.55 and 91.35% respectively. Spirulina CPCC-695 laccase with efficient estrone degradation ability renders them promising candidates for EDCs bioremediation.

通过水生系统在食物链中添加雌酮等外源性内分泌干扰化合物 (EDC)，通过改变基因组或非基因组途径来破坏类固醇的生物合成和代谢，最终导致各种疾病。因此，迫切需要对这些化合物进行生物修复，以防止它们在环境中添加和持久存在。酶降解已被证明是穿着闪亮盔甲的骑士，因为它是安全的并且不会产生有毒产物。多铜氧化酶（EC 1.10.3.2 苯二酚：氧氧化还原酶）、漆酶具有降解酚类和非酚类底物的潜力，最近引起了人们的关注。在本研究中，漆酶被纯化、表征并用于研究雌酮降解。使用冷丙酮浓缩和沉淀培养物滤液（粗漆酶），并用 tris 缓冲液（50 mM）透析，得到四倍部分纯化的形式，产率 45.56%，比活性为 204.14 U/mg，单峰为250–300 纳米。通过 SDS-PAGE 估计，部分纯化的漆酶约为 80 kDa，优选 ABTS 作为底物。粗漆酶和部分纯化的漆酶在 pH 3.0、40 °C 和 4 mM ABTS 下均显示出最大活性。粗制和部分纯化的漆酶的动力学常数（Km、Vmax）为 0.83 mM；494.31mM/分钟和0.58mM；分别为 480.54 mM/min。硫酸铁和叠氮化钠最大程度地抑制漆酶。粗漆酶和半纯化漆酶的降解效率分别为87.55%和91.35%。螺旋藻CPCC-695 漆酶具有高效的雌酮降解能力，使其成为 EDC 生物修复的有希望的候选者。