Endophytic fungi as well as arbuscular mycorrhizal fungi (AMF) are known to stimulate plant growth and production of secondary metabolites in medicinal plants. Here, 10 endophytic fungi isolated from roots of wild Alkanna tinctoria plants and 5 AMF purchased from the Glomeromycota in vitro collection were evaluated, during two successive three-month greenhouse experiments, on the growth of Echium vulgare and alkannin/shikonin and their derivatives (A/Sd) production in the roots. Some of the endophytic fungi tested significantly increased plant growth parameters as compared to the control: Cladosporium allicinum, Cadophora sp., Clonostachys sp., Trichoderma hispanicum and Leptosphaeria ladina increased root volume, Plectosphaerella sp. And T. hispanicum root fresh weight and root water retention and T. hispanicum plant water retention. However, none of these fungi impacted A/Sd production. Conversely, none of the AMF strains tested impacted plant growth parameters, but those inoculated with Rhizophagus intraradices MUCL 49410 had a significantly higher concentration of alkannin/shikonin (A/S), acetyl-A/S, β,β– dimethylacryl-A/S, isovaleryl-A/S and total A/Sd, compared to the control plants. Further studies are needed to investigate the mechanisms involved in the production of A/Sd in plants associated to specific endophytic fungi/AMF and on the cultivation conditions required for optimal production of these compounds.

已知内生真菌和丛枝菌根真菌（AMF）可以刺激药用植物的生长和次生代谢产物的产生。在这里，在两个连续三个月的温室实验中，对从野生Alkannatinctoria植物根部分离的 10 种内生真菌和从肾囊菌门体外收藏中购买的 5 种 AMF 进行了评估，以评估蓝蓟和紫草素/紫草素及其衍生物的生长情况（A /Sd) 根部生产。与对照相比，所测试的一些内生真菌显着提高了植物生长参数：大蒜枝孢菌（Cladosporium allicinum）、Cadophora sp。，Clonostachys sp。、 西班牙木霉 (Trichoderma hispanicum )和Leptosphaeria ladina增加了根体积，Plectosphaerella sp. 增加了根体积。以及T. hispanicum根鲜重和根保水性以及T. hispanicum植物保水性。然而，这些真菌都不影响 A/Sd 的生产。相反，所测试的 AMF 菌株均不影响植物生长参数，但接种根内根瘤菌MUCL 49410 的菌株具有显着更高浓度的阿尔坎宁/紫草素 (A/S)、乙酰基-A/S、β,β- 二甲基丙烯酰基-A/ S、异戊酰-A/S 和总 A/Sd，与对照植物相比。需要进一步研究来调查与特定内生真菌/AMF 相关的植物中 A/Sd 产生的机制，以及最佳产生这些化合物所需的栽培条件。