A series of biodegradable nanoparticle-based drug delivery systems have been designed utilizing poly(β-amino ester)-guanidine-phenylboronic acid (PBAE-G) polymers. In this study, a novel Lentinan-Functionalized PBAE-G-nanodiamond system was developed to carry ovalbumin (LNT-PBAE-G-ND@OVA). The impact of this drug delivery system on the activation and maturation of macrophages was then assessed. Furthermore, LNT-PBAE-G-ND@OVA induced potent antibody response and showed no obvious toxicity in vitro and in vivo. Moreover, treatment with LNT-PBAE-G-ND@OVA was sufficient to alter the expression of genes associated with the cGAS-STING pathway, and the LNT-PBAE-G-ND@OVA induced upregulation of costimulatory molecules. LNT-PBAE-G-ND@OVA treatment was sufficient to induce macrophage activation through a complex mechanism in which cGAS-STING signaling plays an integral role.

利用聚（β-氨基酯）-胍-苯基硼酸（PBAE-G）聚合物设计了一系列可生物降解的基于纳米颗粒的药物递送系统。在本研究中，开发了一种新型香菇多糖功能化PBAE-G-纳米金刚石系统来携带卵清蛋白（LNT-PBAE-G-ND@OVA）。然后评估该药物递送系统对巨噬细胞的激活和成熟的影响。此外，LNT-PBAE-G-ND@OVA 诱导了有效的抗体反应，并且在体外和体内没有表现出明显的毒性。此外，LNT-PBAE-G-ND@OVA 处理足以改变与 cGAS-STING 通路相关的基因表达，并且 LNT-PBAE-G-ND@OVA 诱导共刺激分子上调。LNT-PBAE-G-ND@OVA 处理足以通过复杂的机制诱导巨噬细胞活化，其中 cGAS-STING 信号传导发挥着不可或缺的作用。