Quantitative information on blood metabolites can be used in developing advanced medical strategies such as early detection and prevention of disease. Monitoring bioactive lipids such as steroids, bile acids, and polyunsaturated fatty acid (PUFA) metabolites could be a valuable indicator of health status. However, a method for simultaneously measuring these bioactive lipids has not yet been developed. Here, we report a liquid chromatography tandem mass spectrometry (LC/MS/MS) method that can simultaneously measure 144 bioactive lipids, including steroids, bile acids, and PUFA metabolites, from human plasma, and a sample preparation method for these targets. Protein removal by methanol precipitation and purification of bioactive lipids by solid-phase extraction improved the recovery of the targeted compounds in human plasma samples, demonstrating the importance of sample preparation methods for a wide range of bioactive lipid analyses. Using the developed method, we studied the plasma from healthy human volunteers and confirmed the presence of bioactive lipid molecules associated with sex differences and circadian rhythms. The developed method of bioactive lipid analysis can be applied to health monitoring and disease biomarker discovery in precision medicine.

中文翻译：

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通过 LC/MS/MS 对人血浆中的生物活性脂质进行广泛的靶向分析。

血液代谢物的定量信息可用于制定先进的医疗策略，例如疾病的早期检测和预防。监测类固醇、胆汁酸和多不饱和脂肪酸 (PUFA) 代谢物等生物活性脂质可能是健康状况的重要指标。然而，尚未开发出同时测量这些生物活性脂质的方法。在这里，我们报告了一种液相色谱串联质谱（LC/MS/MS）方法，可以同时测量人血浆中的 144 种生物活性脂质，包括类固醇、胆汁酸和 PUFA 代谢物，以及针对这些目标的样品制备方法。通过甲醇沉淀去除蛋白质和通过固相萃取纯化生物活性脂质提高了人血浆样品中目标化合物的回收率，证明了样品制备方法对于各种生物活性脂质分析的重要性。使用开发的方法，我们研究了健康人类志愿者的血浆，并证实了与性别差异和昼夜节律相关的生物活性脂质分子的存在。所开发的生物活性脂质分析方法可应用于精准医学中的健康监测和疾病生物标志物发现。