Background

The risk factors for the pathogenesis of HCC are highly variable, and the prognosis of patients is very unsatisfactory. In this study, we investigated the regulatory effect of LINC00943 on HCC progression and its relationship with clinicopathological features.

Methods

LINC00943 level in HCC tissues and cell specimens was verified by RT-qPCR. The pathologic significance of LINC00943 in the prognosis of HCC was analyzed by Kaplan-Meier and Cox regression analyses. The behavioral function of LINC00943 in HCC cells was evaluated via CCK-8 and Transwell assays. The specific targeting relationship between LINC00943 and miR-195–5p was investigated by luciferase activity assay.

Results

LINC00943 was highly expressed in HCC tissues and cell specimens. Clinical data analysis showed that elevated LINC00943 indicated poor prognosis in patients with HCC and was related to TNM stage and lymph node metastasis. Cell experiments demonstrated that silencing LINC00943 sponge miR-195–5p suppressed the proliferation, migration and invasion of HCC cells. Mechanistically, miR-195–5p inhibitor remedied the suppressive effect of silencing LINC00943 on the biological functions of HCC cells.

Conclusion

LINC00943 may be an independent prognostic factor of HCC, which provides new thinking for the prognosis and treatment of HCC patients.

中文翻译：

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lncRNA LINC00943对肝细胞癌进展的调控作用及其与临床病理特征的关系

背景

HCC发病的危险因素变化很大，患者的预后很不理想。在本研究中，我们研究了 LINC00943 对 HCC 进展的调节作用及其与临床病理特征的关系。

方法

通过 RT-qPCR 验证 HCC 组织和细胞样本中的 LINC00943 水平。通过 Kaplan-Meier 和 Cox 回归分析分析 LINC00943 在 HCC 预后中的病理意义。通过 CCK-8 和 Transwell 测定评估 LINC00943 在 HCC 细胞中的行为功能。通过荧光素酶活性测定研究了 LINC00943 和 miR-195-5p 之间的特异性靶向关系。

结果

LINC00943 在 HCC 组织和细胞标本中高表达。临床数据分析显示，LINC00943升高提示HCC患者预后不良，且与TNM分期和淋巴结转移相关。细胞实验表明，沉默LINC00943海绵miR-195-5p可抑制HCC细胞的增殖、迁移和侵袭。从机制上讲，miR-195-5p抑制剂可以弥补沉默LINC00943对HCC细胞生物学功能的抑制作用。

结论

LINC00943可能是HCC的独立预后因素，为HCC患者的预后和治疗提供新思路。