Floccularia luteovirens is one of the rare edible fungi with high nutritional value found on the Qinghai-Tibet Plateau. However, research at the molecular level on this species is currently constrained due to the lack of reliable reference genes for this species. Thirteen potential reference genes (ACT, GAPDH, EF-Tu, SAMDC, UBI, CLN1, β-TUB, γ-TUB, GTP, H3, UBC, UBC-E2, and GTPBP1) were chosen for the present study, and their expression under various abiotic conditions was investigated. Stability of gene expression was tested using GeNorm, NormFinder, BestKeeper, Delta–Ct, and RefFinder. The results showed that the most suitable reference genes for salt treatment were ACT and EF-Tu. Under drought stress, γ-TUB and UBC-E2 would be suitable for normalization. Under oxidative stress, the reference genes H3 and GAPDH worked well. Under heat stress, the reference genes EF-Tu and γ-TUB were suggested. Under extreme pH stress, UBC-E2 and H3 were appropriate reference genes. Under cadmium stress, the reference genes ACT and UBC-E2 functioned well. In different tissues, H3 and GTPBP1 were appropriate reference genes. The optimal internal reference genes when analyzing all samples were H3 and SAMDC. The expression level of HSP90 was studied to further validate the applicability of the genes identified in this study.

黄绿绒球菌是青藏高原上珍稀的营养价值极高的食用菌之一。然而，由于该物种缺乏可靠的参考基因，目前对该物种的分子水平研究受到限制。本研究选择了13 个潜在参考基因（ACT、GAPDH、EF-Tu、SAMDC、UBI、CLN1、β-TUB、γ-TUB、GTP、H3、UBC、UBC-E2和GTPBP1 ），及其表达在各种非生物条件下进行了研究。使用 GeNorm、NormFinder、BestKeeper、Delta–Ct 和 RefFinder 测试基因表达的稳定性。结果表明，最适合盐处理的内参基因是ACT和EF-Tu。在干旱胁迫下，γ-TUB和UBC-E2适合标准化。在氧化应激下，内参基因H3和GAPDH工作良好。在热应激下，建议参考基因EF-Tu和γ-TUB 。在极端pH胁迫下，UBC-E2和H3是合适的参考基因。在镉胁迫下，内参基因ACT和UBC-E2功能良好。在不同的组织中，H3和GTPBP1是合适的参考基因。分析所有样品时的最佳内参基因是H3和SAMDC。研究了HSP90的表达水平，以进一步验证本研究中确定的基因的适用性。