Exposure to time-varying electromagnetic fields (EMF) has the capacity to influence biological systems. Our results demonstrate that exposure to time-varying EMF modeled after the physiological firing frequency of intercellular calcium waves can inhibit proliferation and induce apoptosis in malignant cells. Single exposure of B16-BL6 cells to a Ca²⁺ EMF for 40 min reduced the number of viable cells by 50.3%. Cell imaging with acridine orange and ethidium bromide dye revealed substantial cellular apoptosis, preapoptotic cells, nuclear fragmentation, and large spacing between cells in the Ca²⁺ EMF condition when compared to the control condition. The ability of Ca²⁺ EMF to influence the proliferation and survival of malignant cells suggests that exposure to specific EMF may function as a potential anticancer therapy.

中文翻译：

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暴露于细胞间钙波模型电磁场后诱导 B16-BL6 黑色素瘤细胞凋亡

暴露于时变电磁场 (EMF) 能够影响生物系统。我们的结果表明，暴露于以细胞间钙波生理放电频率为模型的时变电磁场可以抑制恶性细胞的增殖并诱导细胞凋亡。B16-BL6 细胞单次暴露于 Ca ²⁺ EMF 40 分钟，活细胞数量减少了 50.3%。与对照条件相比，使用吖啶橙和溴化乙锭染料进行的细胞成像显示，在 Ca ²⁺ EMF条件下，大量细胞凋亡、凋亡前细胞、核碎裂以及细胞间距较大。^{Ca 2+} EMF 影响恶性细胞增殖和存活的能力表明，暴露于特定 EMF 可能作为一种潜在的抗癌疗法。